Anti-Sorting Nexin-5 (SNX5) Monoclonal Antibody for IHC-P and ELISA - Q9Y5X3

Core Product Specifications and Parameters

Biological Background: SNX5 Function and Localization

• Sorting nexin-5 (SNX5) is a component of the retromer membrane-deforming SNX-BAR subcomplex, which mediates retrograde transport of cargo from endosomes to the trans-Golgi network (TGN). SNX5 interacts with membranes containing phosphatidylinositol 3-phosphate (PtdIns(3P)) or phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4)P2). Related references: PMID:15561769
• SNX5 is involved in the retrograde transport of the lysosomal enzyme receptor IGF2R, facilitating its recycling and contributing to lysosomal function. Related references: PMID:17148574 PMID:18596235
• SNX5 plays a role in epidermal growth factor receptor (EGFR) internalization upon EGF stimulation, and participates in EGFR endosomal sorting and degradation via a retromer-independent mechanism that involves PIP5K1C isoform 3. Related references: PMID:23602387
• It regulates E-cadherin sorting and degradation by inhibiting PIP5K1C isoform 3-mediated degradation, thereby impacting cell adhesion dynamics. Related references: PMID:24610942
• SNX5 is also implicated in macropinocytosis, a process essential for non-selective fluid-phase uptake and membrane trafficking. Related references: PMID:18854019 PMID:21048941
• Subcellularly, SNX5 localizes to early endosome membranes, cytoplasmic vesicles, the cell membrane, and cell projections such as phagocytic cups and ruffles, underscoring its role in dynamic membrane remodeling.
• The protein contains a Phox homology (PX) domain that binds phosphoinositides and a BAR domain for sensing membrane curvature. Post-translational modifications include acetylation and phosphorylation, and it has been implicated in host-virus interactions.

Experimental Guidance and Technical Tips

• The immunogen was designed using a recombinant fusion protein spanning amino acids 28–127 of human SNX5, which corresponds to a region potentially exposed and suitable for generating antibodies with specificity to the N-terminal portion of the protein.
• For immunohistochemistry (IHC-P), consider using formalin-fixed paraffin-embedded tissue sections with appropriate antigen retrieval. Start with a recommended dilution range based on endogenous expression levels and validate with positive and negative control tissues.
• The antibody’s reactivity in ELISA suggests it recognizes the immunogen well; when performing ELISA, include purified recombinant SNX5 as a positive control and pre-immune sera as a negative control.
• Although cross-reactivity is confirmed for mouse and rat, it is advisable to validate the antibody in your specific model system, especially if using detection methods beyond IHC-P and ELISA (e.g., Western blotting is not listed as a validated application, so additional verification would be needed).
• For studies involving endosomal trafficking or degradation assays, the antibody may be useful for immunofluorescence or co-immunoprecipitation, but such uses should be empirically validated.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing bridge for IVD raw materials, technical services, and consulting, supporting diagnostic manufacturers and researchers from concept to clinic. Our portfolio includes validated antibody pairs, high-quality monoclonal antibodies like this anti-SNX5 rabbit mAb, and bulk ancillary reagents. Whether you require specific antibodies for intracellular trafficking research or rare target raw-material sourcing, CamelBio is equipped to streamline your supply chain.