Anti-Sorbitol Dehydrogenase Rabbit Polyclonal Antibody for WB, IF/ICC, IHC-P, ELISA - Q00796

Core Product Specifications and Parameters

Biological Background: Sorbitol Dehydrogenase Function and Localization

• Sorbitol dehydrogenase (SORD) is a polyol dehydrogenase that catalyzes the reversible NAD-dependent oxidation of various sugar alcohols, with highest activity toward D-sorbitol, L-threitol, xylitol, and ribitol, converting them to D-fructose, L-erythrulose, D-xylulose, and D-ribulose, respectively. Related references: PMID:3365415
• SORD is a key enzyme in the polyol pathway, which interconverts glucose and fructose via sorbitol. This pathway is implicated in the pathogenesis of diabetic complications such as neuropathy and retinopathy under hyperglycemic conditions. Related references: PMID:12962626 PMID:25105142 PMID:29966615
• The enzyme also catalyzes the stereospecific oxidation of (2R,3R)-2,3-butanediol and, to a lesser extent, can oxidize L-arabinitol, galactitol, D-mannitol, and glycerol in vitro, but it cannot use NADP+ as a cofactor. Related references: PMID:3365415
• Subcellularly, SORD localizes to the mitochondrial membrane and is also found in cell projections such as cilia and flagella.
• Tissue expression is prominent in the liver, kidney, and epithelial cells of both benign and malignant prostate tissue, as well as in the epididymis at the protein level.
• Post-translational modifications include acetylation and phosphorylation, and the protein exists in alternatively spliced isoforms.
• SORD may play a role in sperm motility by utilizing sorbitol as an alternative energy source. Related references: PMID:16278369

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 145-300 of human SORD (NP_003095.2), targeting a region distal to the N-terminal mitochondrial targeting sequence; this may facilitate detection of the mature mitochondrial form.
• For Western blot, the predicted molecular weight of the full-length precursor is ~38 kDa, while the processed mitochondrial form may appear smaller; validate band size with appropriate positive controls and lysates.
• For IHC-P, consider antigen retrieval methods optimized for mitochondrial membrane proteins, and include appropriate tissue controls (e.g., liver, kidney).
• Cross-reactivity is predicted with mouse and rat based on sequence homology; initial species-specific validation is recommended in the relevant sample system.
• As a polyclonal antibody, lot-to-lot consistency may vary; titration and optimization are advised for each new application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive access to IVD raw materials, supporting diagnostic manufacturers and research labs from concept to clinic. This anti-Sorbitol Dehydrogenase polyclonal antibody addresses key targets in the polyol pathway and diabetic complication research. Leverage our validated antibody pairs, bulk ancillary reagents, and rare target sourcing capabilities to streamline your assay development and procurement needs.