Anti-Sonic Hedgehog (Shh) Rabbit Polyclonal Antibody for WB, IF/ICC, IF-P, IHC-P, ELISA - Q15465

Core Product Specifications and Parameters

Biological Background: Sonic Hedgehog (Shh) Function and Localization

• Sonic hedgehog protein (SHH) is a developmental morphogen that is synthesized as a precursor protein. The precursor undergoes autoproteolytic cleavage and cholesterol transfer in the endoplasmic reticulum to generate the N-terminal signaling domain (ShhN) and the C-terminal domain (ShhC). Related references: PMID:29954986 PMID:29995851 PMID:30139912
• The dually lipidated ShhN acts as a morphogen by binding to the patched receptor (PTCH1/PTCH2), which relieves inhibition of smoothened (SMO), thereby activating the SMO signaling pathway and transcription of target genes. Related references: PMID:10753901 PMID:24522195 PMID:24863049
• Shh is essential for a variety of patterning events during development, including central nervous system patterning (ventral cell fate induction), limb development (anterior-posterior axis), and axon guidance.
• Subcellular localization includes the endoplasmic reticulum membrane, Golgi apparatus membrane, and secretion to the cell surface; ShhC is degraded in the ER.
• The protein is associated with several diseases when mutated, notably holoprosencephaly and microphthalmia (as indicated by UniProt keywords).
• Additional keywords: calcium-binding, zinc-binding, glycoprotein, palmitoylated, lipoprotein, hydrolase, transferase, and reference proteome.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 200–300 of human Shh; consider this region when interpreting reactivity with different isoforms or cleavage products.
• For Western blotting, the full-length protein is predicted at ~50 kDa, but processed forms may be detected; perform appropriate controls.
• In immunohistochemistry and immunofluorescence, antigen retrieval and permeabilization may be required due to membrane association; validate in the relevant tissue.
• For ELISA applications, the peptide immunogen may serve as a positive control; cross-reactivity with mouse and rat suggests utility across species.
• Optimize antibody dilution in each application using a known positive sample.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing bridge for IVD raw materials, offering reliable antibodies like this anti-Shh polyclonal antibody to support developmental biology and oncology research. Our portfolio includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to accelerate diagnostic development from concept to clinic.