Anti-Sonic Hedgehog (Shh) Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q15465

Core Product Specifications and Parameters

Biological Background: Sonic Hedgehog (Shh) Function and Localization

• Sonic hedgehog protein (Shh), encoded by the SHH gene, is a morphogen precursor with aliases including HHG-1 and ShhNC. It belongs to the hedgehog family of developmental signaling molecules.
• The full-length precursor undergoes autoproteolysis and cholesterol transferase activity in the endoplasmic reticulum, cleaving into the N-terminal signaling domain (ShhN) and C-terminal fragment (ShhC), with cholesterol attached to ShhN. Related references: PMID:29954986 PMID:29995851 PMID:30139912
• The dually lipidated ShhN acts as a morphogen by binding to the Patched receptor (PTCH1/PTCH2), relieving inhibition of Smoothened (SMO), thereby activating the SMO signaling pathway and downstream target gene transcription. Related references: PMID:10753901 PMID:24522195 PMID:24863049 PMID:29995851 PMID:31548691
• Shh is essential for multiple developmental processes, including central nervous system patterning, limb bud anterior-posterior axis formation, neural tube and somite ventral cell fate specification, and axon guidance.
• Subcellularly, Shh localizes to the endoplasmic reticulum membrane, Golgi apparatus membrane, and is secreted to the extracellular space; it also associates with the cell membrane via its lipid modifications.
• Key keywords include autocatalytic cleavage, calcium-binding, developmental protein, disease-associated variants (holoprosencephaly, microphthalmia), glycoprotein, lipoprotein, and zinc-binding.

Experimental Guidance and Technical Tips

• Given the processing of Shh precursor into ShhN and ShhC, Western blotting may detect multiple bands; antibodies raised against the C-terminal region (aa 231-330) may recognize both the full-length precursor (~50 kDa) and possibly processed fragments.
• For immunofluorescence/immunocytochemistry, signal may be observed at the plasma membrane, Golgi apparatus, or endoplasmic reticulum, reflecting the protein's trafficking and secretion. Co-staining with organelle markers is recommended for precise localization.
• This polyclonal antibody, produced in rabbit with a synthetic peptide immunogen, may exhibit lot-to-lot variability; include appropriate positive and negative controls in each experiment.
• While validated for human samples, cross-reactivity with other species has not been tested; verify reactivity in the relevant model system if using non-human samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, supporting diagnostic developers and researchers from concept to clinic. For investigations into the hedgehog signaling pathway and developmental biology, we supply validated antibodies such as this anti-Shh polyclonal antibody, alongside optimized monoclonal/polyclonal antibody pairs, bulk ancillary reagents, and rare target raw-material sourcing. Our portfolio is designed to streamline your assay development and scale-up with reliable, high-quality bioreagents.