Anti-Sonic Hedgehog (Shh) Monoclonal Antibody for WB, IF/ICC, IF-P, ELISA - Q15465

Core Product Specifications and Parameters

Biological Background: Sonic Hedgehog (Shh) Function and Localization

• Sonic hedgehog (SHH) is synthesized as a precursor protein that undergoes autoproteolytic cleavage and cholesterol modification to generate the active N-terminal signaling domain (ShhN) and a C-terminal fragment (ShhC). Related references: PMID:29954986, PMID:29995851, PMID:30139912
• The dually lipidated ShhN morphogen binds to the Patched receptor (PTCH1/PTCH2), relieving inhibition of Smoothened (SMO) and activating downstream target gene transcription. Related references: PMID:10753901, PMID:24522195, PMID:24863049, PMID:29995851, PMID:31548691
• SHH localizes to the endoplasmic reticulum and Golgi membrane during processing; the mature, secreted ShhN can associate with the cell membrane.
• SHH plays critical roles in embryonic development, including central nervous system patterning, limb bud axis specification, and axon guidance.
• The protein contains a zinc-binding protease domain and exhibits hydrolase and transferase activities, reflecting its autocatalytic cleavage and cholesterol transfer functions.
• Mutations in SHH are associated with developmental disorders such as holoprosencephaly and microphthalmia.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 200-300 of human SHH; this region may be conserved across species, consistent with observed cross-reactivity.
• For Western blotting, the predicted full-length molecular weight is 50 kDa, but processed forms may appear at lower molecular weights; validate with appropriate controls.
• In immunofluorescence applications (IF/ICC, IF-P), optimize fixation and permeabilization conditions for the specific cell or tissue type, and confirm localization patterns relative to published data.
• For ELISA, consider using paired antibodies or recombinant protein standards to establish assay sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and life science labs with high-quality IVD raw materials, including validated monoclonal antibodies like this anti-Sonic Hedgehog (Shh) antibody for developmental biology and oncology research. We supply optimized antibody pairs, bulk ancillary reagents, and support one-stop sourcing for rare or specialized targets to streamline your assay development from concept to clinic.