Anti-SON Rabbit Polyclonal Antibody (WB/ELISA) - P18583

Core Product Specifications and Parameters

Biological Background: SON Function and Localization

• SON is a large RNA-binding protein also known as BASS1, DBP-5, NREBP, and SON3, encoded by the SON gene (C21orf50, KIAA1019).
• It functions as an mRNA splicing cofactor, promoting efficient splicing of transcripts with weak splice sites, particularly cell-cycle and DNA-repair genes such as TUBG1, KATNB1, AURKB, and AKT1.
• SON facilitates interaction between serine/arginine-rich splicing factors (e.g., SRSF2) and RNA polymerase II to enhance spliceosome assembly.
• It also binds DNA, recognizing the consensus sequence 5'-GA[GT]AN[CG][AG]CC-3', and may repress hepatitis B virus (HBV) core promoter activity.
• Essential for correct RNA splicing of genes critical for brain development and neuronal migration, including TUBG1, FLNA, PNKP, and WDR62 Related references: PMID:27545680
• Subcellularly localized to nuclear speckles, consistent with its role in pre-mRNA processing.
• Widely expressed across tissues, with higher levels in leukocytes and heart.
• Post-translational modifications include acetylation, phosphorylation, methylation, and ubiquitination; the protein is also subject to alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a sequence within amino acids 850–950 of human SON (NP_001278340.1). This region may be conserved across isoforms; confirm specificity by knockdown or knockout controls.
• For Western blot, consider using a low-percentage gel (e.g., 6–8%) and extended transfer times to accommodate the large molecular weight (~264 kDa).
• SON localizes to nuclear speckles; enriching for nuclear fractions may improve detection in WB and ELISA.
• Validate antibody performance in biologically relevant sample systems, such as cell lines with high SON expression (e.g., Jurkat or HeLa) or tissues like heart.
• For functional studies, consider coupling with assays that measure splicing efficiency or cell-cycle progression.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with reliable access to validated raw materials, including this high-quality anti-SON polyclonal antibody. Whether you need optimized antibody pairs, bulk ancillary reagents, or assistance sourcing rare-target raw materials for splicing and cell-cycle studies, our integrated supply chain supports your workflow from concept to clinic.