Anti-Somatotropin (GH1) Polyclonal Antibody for WB, IF-P, IHC-P, ELISA - P01241

Core Product Specifications and Parameters

Biological Background: Somatotropin (GH1) Function and Localization

• GH1 encodes somatotropin, also known as growth hormone (GH), a key pituitary hormone essential for postnatal growth and metabolic regulation.
• It acts by binding to the growth hormone receptor, primarily stimulating the liver and other tissues to produce insulin-like growth factor 1 (IGF1), which mediates many of its anabolic and growth-promoting effects.
• GH1 directly promotes the differentiation and proliferation of myoblasts and enhances amino acid uptake and protein synthesis in muscle and other tissues.
• The protein is secreted from the anterior pituitary into the systemic circulation, functioning as an endocrine hormone.
• Post-translational modifications include phosphorylation, and the hormone contains two conserved disulfide bonds that stabilize its four-helix bundle structure.
• Clinical relevance is underscored by disease variants linked to growth disorders, such as isolated growth hormone deficiency (IGHD) and dwarfism.
• Keywords associated with GH1 include hormone, secreted, disulfide bond, metal-binding (zinc), phosphoprotein, and disease variant.

Experimental Guidance and Technical Tips

• The immunogen for this antibody is a synthetic peptide spanning amino acids 100-200 of human GH1 (NP_000506.2). Consider using the immunizing peptide in blocking experiments to confirm signal specificity.
• Based on the recommended applications (WB, IF-P, IHC-P, ELISA), this antibody is suitable for detection of endogenous and recombinant GH1 across multiple assay formats. Validate the optimal working dilution and protocol in your specific sample system.
• The predicted molecular weight of the GH1 proprotein is approximately 25 kDa (mature form ~22 kDa). Use appropriate molecular weight markers and take into account potential signal peptide cleavage when interpreting band patterns.
• Reported cross-reactivity includes human, mouse, and rat. When using samples from other species, verify reactivity and adjust experimental conditions accordingly.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD-grade antibodies and ancillary reagents, supporting diagnostic manufacturers and researchers from concept to clinic. This anti-GH1 polyclonal antibody is part of our catalog for growth hormone and endocrine-related assay development. We also provide validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk reagents, and sourcing of rare-target raw materials to streamline your IVD workflow.