Anti-SOGA1 Rabbit Polyclonal Antibody for WB, ELISA - O94964

Core Product Specifications and Parameters

Biological Background: SOGA1 (MTCL2) Function and Localization

• SOGA1, also known as Microtubule cross-linking factor 2 (MTCL2) or SOGA family member 1, is a large protein encoded by the MTCL2 gene in humans (GeneID: 140710). It belongs to the SOGA family and plays key roles in microtubule dynamics and autophagy.
• Acts as a microtubule-associated factor that integrates centrosomal and Golgi-associated microtubules on the Golgi membrane, thereby supporting directional cell migration. The N-terminal coiled-coil region mediates Golgi membrane association, while the C-terminal domain directly binds microtubules (By similarity).
• Required for faithful chromosome segregation during mitosis, highlighting its essential role in cell cycle regulation (PubMed:33587225). Related references: PMID:33587225
• Involved in the regulation of autophagy by playing a role in the reduction of glucose production in an adiponectin- and insulin-dependent manner (By similarity).
• Subcellularly, SOGA1 localizes to the cytoplasm, cytoskeleton, Golgi apparatus membrane, and midbody, and is also detected as a secreted protein (UniProtKB O94964).
• Features several functional motifs including coiled-coil regions, and is subject to phosphorylation and alternative splicing, as indicated by UniProt keywords.

Experimental Guidance and Technical Tips

• The immunogen for this antibody was designed using a recombinant fusion protein corresponding to amino acids 650-750 of human SOGA1 (NP_542194.2). This region may provide specificity for detecting SOGA1 isoforms across multiple species.
• For Western blotting (WB), a band around 160 kDa is expected, consistent with the predicted molecular weight. However, due to potential post-translational modifications, slight variations may occur. Validate the observed band size in the relevant sample system.
• For ELISA applications, optimize coating antigen and antibody dilutions using standard indirect ELISA protocols.
• Cross-reactivity with mouse and rat SOGA1 allows for comparative studies across species; however, users should confirm reactivity in their specific experimental models.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, providing reliable polyclonal antibodies like this anti-SOGA1 antibody to researchers and diagnostic manufacturers. Our portfolio supports essential research areas such as autophagy, cell cycle, and microtubule dynamics. Through custom antibody development, validated antibody pairs, and bulk reagent supply, CamelBio helps streamline your development pipeline from concept to clinical application.