Anti-SOD2 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P04179

Core Product Specifications and Parameters

Biological Background: Superoxide Dismutase [Mn], Mitochondrial Function and Localization

• SOD2 encodes superoxide dismutase [Mn], a mitochondrial matrix enzyme that catalyzes the dismutation of superoxide radicals into oxygen and hydrogen peroxide, serving as a primary antioxidant defense.
• The enzyme requires manganese as a cofactor for its catalytic activity, as indicated by the Metal-binding keyword.
• SOD2 is synthesized as a precursor with a transit peptide, which directs its import into mitochondria, where it is cleaved to yield the mature active form (supported by the Transit peptide keyword).
• Subcellularly, SOD2 localizes specifically to the mitochondrial matrix, consistent with its role in protecting mitochondria from oxidative damage.
• The protein is subject to various post-translational modifications, including acetylation, nitration, and ubiquitin-like conjugation (from keywords: Acetylation, Nitration, Ubl conjugation), which may modulate its activity, stability, or signaling functions.
• Alternative splicing of the SOD2 gene produces multiple transcript variants (from keyword Alternative splicing), potentially contributing to isoform diversity and tissue-specific regulation.
• As a critical antioxidant enzyme, SOD2 is implicated in cellular responses to oxidative stress, with dysregulation linked to various diseases including neurodegenerative disorders and cancer.

Experimental Guidance and Technical Tips

• The antibody was raised against a recombinant fragment corresponding to amino acids 25-222, which covers the mature region of SOD2; it may detect both the precursor and mature forms depending on sample preparation and epitope exposure.
• For Western blotting, the antibody is expected to detect a band at approximately 25 kDa corresponding to the mature SOD2 protein; in some cell or tissue lysates, a higher molecular weight band representing the precursor may also be observed.
• In immunohistochemistry and immunofluorescence, mitochondrial staining patterns should be verified; consider co-staining with a mitochondrial marker to confirm specificity.
• Pre-validation of antigen retrieval and fixation conditions is recommended for optimal IHC-P or IF/ICC results, as SOD2 localization can be affected by processing.
• ELISA protocols can be developed using the recombinant immunogen as a standard, but cross-reactivity with other SOD isoforms (e.g., SOD1, SOD3) should be evaluated to ensure assay specificity.
• Given the high sequence homology among human, mouse, and rat SOD2, the antibody is expected to cross-react with all three species as indicated; however, it is advisable to confirm reactivity in the specific experimental system.

CamelBio: Your One-Stop Sourcing Bridge

As a leading IVD raw material supplier, CamelBio provides comprehensive access to high-quality antibodies, including this anti-SOD2 polyclonal antibody for oxidative stress research. Beyond this product, we support diagnostic manufacturers and laboratories with validated antibody pairs, optimized recombinant proteins, and bulk ancillary reagents to accelerate assay development from discovery to clinical application. For specialized needs, CamelBio also offers rare target raw-material sourcing and technical consulting to bridge critical gaps in your diagnostic pipeline.