Anti-SOD2 Monoclonal Antibody [KO Validated] for WB, IF-P, IHC-P, ELISA - P04179

Core Product Specifications and Parameters

Biological Background: SOD2 (MnSOD) Function and Localization

• SOD2 encodes the mitochondrial manganese-dependent superoxide dismutase, a key antioxidant enzyme that eliminates superoxide anion radicals generated during respiration.
• The enzyme functions as a homotetramer, binding one manganese ion per subunit, which is essential for its catalytic activity.
• SOD2 is located in the mitochondrial matrix, protecting the organelle from oxidative damage and influencing cellular reactive oxygen species (ROS) balance.
• Alternative splicing produces multiple transcript variants, though the full-length protein (25 kDa) is the primary functional form.
• Post-translational modifications include acetylation, nitration, and ubiquitin-like conjugation, which may modulate enzyme activity, stability, or localization.
• As a member of the iron/manganese superoxide dismutase family, SOD2 plays a role in aging, cancer, and neurodegenerative diseases.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 1-100, a region conserved across human, mouse, and rat, supporting reported cross-reactivity.
• KO validation suggests the antibody can recognize endogenous SOD2 and confirm target specificity in knockout models.
• For Western blot, consider an initial dilution range typical for monoclonal antibodies; adjust based on sample and detection system.
• For IHC-P and IF-P, optimize antigen retrieval and blocking conditions to reduce nonspecific binding in tissue sections.
• Validate performance in your specific experimental system; pilot testing with positive and negative controls is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw material procurement for diagnostic manufacturers and research labs. This SOD2 antibody supports mitochondrial oxidative stress studies and can be integrated into validated antibody pairs or sandwich ELISA platforms. We also source rare-target reagents, bulk ancillary materials, and offer technical insights to help accelerate your assay development from concept to clinic.