Anti-SOD1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - P00441

Core Product Specifications and Parameters

Biological Background: SOD1 Function and Localization

• SOD1 is a copper-zinc superoxide dismutase that destroys superoxide radicals normally produced within cells, protecting against oxidative damage. Related references: PMID:18948262 PMID:24140062
• It also functions as a hydrogen sulfide oxidase, catalyzing the oxidation of H2S to sulfate, thereby detoxifying H2S and limiting reactive sulfur species accumulation. Related references: PMID:36630448
• SOD1 is primarily localized in the cytoplasm and nucleus.
• The protein belongs to the Cu-Zn superoxide dismutase family and forms a homodimer.
• Post-translational modifications include acetylation, phosphorylation, palmitoylation, and disulfide bond formation, which may regulate its activity and localization.
• Mutations in SOD1 are associated with amyotrophic lateral sclerosis (ALS), a neurodegenerative disease.
• SOD1 requires copper and zinc ions as cofactors for its enzymatic activity.
• The protein is widely studied as an antioxidant and redox regulator, with additional roles in sulfur metabolism.

Experimental Guidance and Technical Tips

• The immunogen comprises virtually the full-length mature protein (amino acids 2-154), so the antibody may recognize both native and denatured SOD1. For WB, expect a band at approximately 16 kDa under reducing conditions.
• For IHC-P, consider antigen retrieval steps and validate staining in known SOD1-positive tissues (e.g., liver, kidney) to confirm specificity.
• In IF/ICC, optimize fixation and permeabilization conditions to detect cytoplasmic and nuclear SOD1 pools.
• For IP, this polyclonal antibody may be suitable; however, confirm enrichment and co‑immunoprecipitation efficiency in the relevant sample system.
• The antibody cross-reacts with mouse and rat SOD1 (as indicated), making it appropriate for rodent model studies. Validate in the relevant sample system before quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated partner for IVD raw materials, supplying high-quality antibodies such as this anti-SOD1 polyclonal antibody to support oxidative stress, neurodegenerative disease, and antioxidant research. In addition to validated antibodies, we provide bulk ancillary reagents and custom sourcing for rare targets, helping streamline your assay development from concept to clinic. Let CamelBio bridge the gap between your research needs and reliable raw-material supply.