Anti-SOD1 Rabbit Monoclonal Antibody for WB, ELISA - P00441

Core Product Specifications and Parameters

Biological Background: SOD1 Function and Localization

• Superoxide dismutase [Cu-Zn] (SOD1, also known as hSod1, hydrogen sulfide oxidase) is a key antioxidant enzyme that catalyzes the dismutation of superoxide radicals into oxygen and hydrogen peroxide, protecting cells from oxidative stress. Related references: PMID:18948262, PMID:24140062
• Additionally, SOD1 functions as a hydrogen sulfide oxidase, catalyzing the oxidation of H₂S to sulfate and preventing the buildup of toxic reactive sulfur species. Related references: PMID:36630448
• SOD1 is predominantly localized in the cytoplasm and nucleus, where it exerts its protective activities.
• Mutations in the SOD1 gene are associated with amyotrophic lateral sclerosis (ALS), a progressive neurodegenerative disorder.
• The protein is a homodimer that binds one copper and one zinc ion per subunit, which are essential for its enzymatic activity and structural stability.
• Post‑translational modifications include acetylation, phosphorylation, ubiquitination, and palmitoylation, which may modulate its function and localization.
• The mature human SOD1 protein has a molecular weight of approximately 15.9 kDa, consistent with the observed 16 kDa band in western blotting.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 2–154 of human SOD1 (nearly full‑length), so the antibody can recognize both native and denatured conformations.
• For western blotting, consider testing initial dilutions in the range of 1:500–1:5000; use human cell or tissue lysates as positive controls.
• In ELISA, the antibody may be used for detection; pairing with a suitable capture antibody is advised for sandwich formats.
• Because SOD1 is highly conserved across species, verify cross‑reactivity if working with non‑human samples.
• Use a 12% or 15% SDS‑PAGE gel for optimal resolution of the ~16 kDa protein.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic developers and research laboratories with seamless access to essential IVD raw materials. This anti-SOD1 rabbit monoclonal antibody supports studies on oxidative stress, neurodegeneration, and related pathways. Beyond individual antibodies, we supply validated antibody pairs, optimized monoclonal/polyclonal reagents, bulk ancillary materials, and can assist in sourcing rare‑target raw materials to accelerate your projects from concept to clinic.