Anti-SOCS6 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - O14544

Core Product Specifications and Parameters

Biological Background: SOCS6 Function and Localization

• SOCS6 (Suppressor of cytokine signaling 6) is a member of the SOCS family proteins that function as classical negative feedback inhibitors of cytokine signal transduction.
• SOCS6 contains an SH2 domain and a SOCS box, and is also known as CIS-4 (Cytokine-inducible SH2 protein 4) and SOCS-4.
• It acts as a substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex, mediating ubiquitination and subsequent proteasomal degradation of target proteins (By similarity).
• SOCS6 specifically regulates KIT receptor degradation by ubiquitinating the tyrosine-phosphorylated receptor, thereby modulating downstream signaling pathways.
• The protein is implicated in growth regulation and signal transduction inhibition, with its activity dependent on the ubiquitin conjugation pathway.
• According to UniProt, the human SOCS6 protein has a calculated molecular weight of approximately 60 kDa (59528 Da).
• It is classified under keywords including 3D-structure, growth regulation, SH2 domain, signal transduction inhibitor, and Ubl conjugation pathway.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-210) of human SOCS6; therefore, the antibody may recognize the full-length protein and potentially N-terminal isoforms or fragments.
• For Western blotting, a band around 60 kDa is expected in human and mouse lysates; consider validating the expression in relevant cell lines or tissues.
• For immunohistochemistry, antigen retrieval methods and antibody dilution should be optimized for the specific tissue type; use appropriate positive and negative controls.
• In ELISA, the antibody can be used for detection of SOCS6 protein; pairing with a monoclonal capture antibody may improve assay performance.
• Cross-reactivity with human and mouse SOCS6 allows for translational studies; however, verify reactivity in other species if required.
• Always include negative controls (e.g., SOCS6 knockout lysates) to confirm antibody specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive range of IVD raw materials and technical support for cytokine signaling research, including validated antibody pairs and optimized polyclonal antibodies like this anti-SOCS6 pAb. We also supply bulk ancillary reagents and facilitate sourcing of rare-target raw materials to accelerate your diagnostic development from concept to clinic.