Anti-SOCS5 Polyclonal Antibody for WB, IHC-P, ELISA - O75159

Core Product Specifications and Parameters

Biological Background: SOCS5 Function and Localization

• SOCS5 (Suppressor of cytokine signaling 5) is a member of the SOCS family, with aliases including CIS6, CISH6, and SOCS-5.
• It functions as a classical negative feedback regulator of cytokine signal transduction, inhibiting pathways such as IL-4, IL-6, and LIF.
• SOCS5 may act as a substrate-recognition component of a SCF-like ECS E3 ubiquitin ligase complex, mediating ubiquitination and proteasomal degradation of target proteins like EGFR.
• By inhibiting IL-4 signaling, SOCS5 plays a role in regulating T-helper cell differentiation, specifically suppressing Th2 polarization.
• The protein contains an SH2 domain and a SOCS box, characteristic of the SOCS family, which are critical for its inhibitory and ubiquitination functions.
• SOCS5 is involved in growth regulation and has been identified in proteomics studies.
• The predicted molecular weight is approximately 61 kDa (UniProt: 61246 Da).

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal 140 amino acids of SOCS5; consider this region's conservation when predicting cross-reactivity.
• For Western blot, a band around 61 kDa is expected; validate with appropriate positive and negative controls.
• In IHC-P, optimize antigen retrieval conditions and antibody dilution based on tissue type.
• This antibody may recognize both human and mouse SOCS5 as indicated; validate in the relevant species sample systems.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers this SOCS5 polyclonal antibody as part of a comprehensive portfolio for diagnostic and research applications. We support manufacturers and labs with validated antibody pairs, optimized antibodies, and bulk reagents tailored to cytokine signaling studies. From concept to clinic, CamelBio provides one-stop access to IVD raw materials, technical services, and consulting.