Anti-SOCS3 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - O14543

Core Product Specifications and Parameters

Biological Background: SOCS3 Function and Localization

• SOCS3 is a member of the suppressor of cytokine signaling (SOCS) family, which forms a classical negative feedback loop regulating JAK/STAT cytokine signal transduction. Related references: PMID:16459330

• It inhibits signal transduction by binding to phosphorylated tyrosine residues on transmembrane receptors such as gp130, LEPR, and G-CSF-R, and directly inhibits JAK2 kinase activity. Related references: PMID:16459330

• SOCS3 functions as a probable substrate recognition component of an SCF-like ECS (Elongin BC-CUL2/5-SOCS-box) E3 ubiquitin-protein ligase complex, mediating ubiquitination and proteasomal degradation of target proteins. Related references: PMID:15601820

• Negatively regulates cytokine signaling through the type II OSM receptor complex by interacting directly with JAK1 upon OSM stimulation. Related references: PMID:16459330

• Widely expressed with high levels in heart, placenta, skeletal muscle, peripheral blood leukocytes, fetal and adult lung, and fetal liver and kidney; lower levels in thymus.

• Contains an SH2 domain and a SOCS box domain, characteristic of the SOCS family, and is classified as a phosphoprotein involved in signal transduction inhibition.

• Suppresses fetal liver erythropoiesis and regulates onset and maintenance of Th2-mediated allergic responses.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 101–225 of human SOCS3; the antibody is expected to recognize full-length endogenous SOCS3 across human, mouse, and rat.
• For Western blot (WB), a band at approximately 25 kDa is expected; consider optimizing antibody dilution and running appropriate controls.
• For IF/ICC, validate fixation and permeabilization conditions in the relevant cell or tissue samples.
• For ELISA, pair with a suitable detection antibody and optimize antigen coating concentration.
• Always include positive and negative control samples to confirm specificity in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, including this high-quality SOCS3 polyclonal antibody. Our comprehensive supply capabilities cover validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing—supporting your JAK/STAT signaling research and assay development from concept to clinic. Partner with CamelBio to streamline your procurement and accelerate your diagnostic or research projects.