Anti-SOCS2 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - O14508

Core Product Specifications and Parameters

Biological Background: SOCS2 Function and Localization

• SOCS2 (Suppressor of cytokine signaling 2), also known as CIS2, SSI2, or STATI2, belongs to the SOCS family of cytokine-inducible SH2-containing proteins.
• SOCS2 functions as the substrate-recognition component of a cullin-5-RING E3 ubiquitin-protein ligase complex (ECS/CRL5). Through its SH2 domain, it binds phosphorylated substrates such as the growth hormone receptor (GHR) and erythropoietin receptor (EPOR), mediating their ubiquitination and subsequent proteasomal degradation, thereby acting as a critical negative regulator of growth hormone and cytokine signaling. Related references: PMID:11781573 PMID:21980433 PMID:25505247 PMID:31182716 PMID:34857742
• SOCS2 is predominantly localized in the cytoplasm.
• It is highly expressed in the heart, placenta, lung, kidney, and prostate, with particularly strong expression in pulmonary type II pneumocytes.
• Proteomics studies indicate that SOCS2 undergoes post-translational modifications, including phosphorylation and ubiquitin conjugation (involving an isopeptide bond), which may regulate its stability and function.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 1-198 of human SOCS2 (NP_001257400.1), representing the N-terminal region prior to the SH2 domain. This design may allow recognition of full-length and potentially truncated or processed forms of the protein.
• For western blotting (WB), expect a band around 22 kDa corresponding to SOCS2. Use appropriate loading controls and verify specificity with lysates from SOCS2-overexpressing or knockdown cell lines where possible.
• For immunofluorescence/ICC, the antibody targets a cytoplasmic protein; optimize fixation (e.g., 4% PFA) and permeabilization (e.g., 0.1% Triton X-100) to ensure proper antigen accessibility.
• For ELISA applications, consider the antibody as a capture or detection reagent after confirming reactivity with your recombinant antigen or native protein. Titrate the antibody to determine optimal working concentration.
• As a polyclonal antibody, it may vary between lots; validate each new lot in your specific assay before routine use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For your SOCS2-related projects, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. Let us streamline your development workflow with reliable, cost-effective supply solutions.