Anti-SOCS2 Monoclonal Antibody for WB, IHC-P, ELISA - O14508

Core Product Specifications and Parameters

Biological Background: SOCS2 Function and Localization

• Suppressor of cytokine signaling 2 (SOCS2), also known as CIS-2, SSI-2, or STATI2, is a member of the SOCS box protein family that functions as an E3 ubiquitin ligase substrate-recognition component.
• SOCS2 is the substrate-recognition component of a cullin-5-RING E3 ubiquitin-protein ligase complex (ECS/CRL5), mediating ubiquitination and subsequent proteasomal degradation of target proteins such as EPOR and GHR. Related references: PMID:11781573, PMID:21980433, PMID:25505247
• Through its SH2 domain, SOCS2 specifically recognizes and binds phosphorylated proteins, promoting their ubiquitination and degradation. Related references: PMID:21980433, PMID:25505247, PMID:31182716
• The ECS(SOCS2) complex acts as a key regulator of growth hormone receptor (GHR) levels by mediating GHR ubiquitination and degradation following JAK2-mediated phosphorylation. Related references: PMID:21980433, PMID:25505247, PMID:34857742
• SOCS2 also catalyzes ubiquitination and degradation of JAK2-phosphorylated erythropoietin receptor (EPOR). Related references: PMID:11781573
• Subcellular localization is primarily cytoplasmic.
• Tissue expression is high in heart, placenta, lung, kidney, and prostate, with predominant expression in pulmonary epithelial cells, specifically type II pneumocytes.
• Keywords: SH2 domain, signal transduction inhibitor, growth regulation, phosphoprotein.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (aa 1-198) of human SOCS2, which includes the SH2 domain critical for phosphotyrosine-dependent substrate recognition; consider this when designing detection or blocking strategies.
• For Western blot (WB), the predicted molecular weight is approximately 22 kDa; include appropriate positive and negative controls to confirm specificity.
• This antibody has been reported for IHC-P; antigen retrieval conditions should be optimized for the specific tissue type.
• In ELISA applications, use standard sandwich or indirect formats and validate with recombinant SOCS2 protein to assess sensitivity.
• Cross-reactivity is confirmed with human and rat samples; verify performance in the relevant sample system before quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For SOCS2-related research in cytokine signaling and growth hormone regulation, CamelBio offers validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. Partner with us to streamline your development pipeline and secure high-quality raw materials for robust immunoassay performance.