Anti-SOCS1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - O15524

Core Product Specifications and Parameters

Biological Background: SOCS1 Function and Localization

• SOCS1 (Suppressor of cytokine signaling 1), also known as JAB, SSI-1, or TIP-3, is a critical negative regulator of cytokine signaling pathways.
• It inhibits the JAK/STAT signaling pathway by binding to JAK kinases and cytokine receptors, thereby suppressing signaling of type I/II interferons, IL-2, IL-4, IL-6, and leukemia inhibitory factor (LIF).
  Related references:
  PMID:32499645
  PMID:33087723
• In vitro, SOCS1 suppresses Tec protein-tyrosine kinase activity.
  Related references:
  PMID:9341160
• Acts as a substrate recognition component of an ECS E3 ubiquitin ligase complex, mediating ubiquitination and subsequent proteasomal degradation of target proteins.
  Related references:
  PMID:11278610
  PMID:11313480
• Contains an SH2 domain and a SOCS box, and localizes to the nucleus and cytoplasmic vesicles.
• Expressed in all tissues with highest levels in spleen, small intestine, and peripheral blood leukocytes.
• Associated with growth regulation and autoinflammatory diseases; disease variants are documented.

Experimental Guidance and Technical Tips

• For Western blotting, the expected band size is approximately 24 kDa. Validate expression in relevant cell lines or tissues, such as Jurkat or HeLa cells, with appropriate controls.
• In immunohistochemistry (IHC-P), consider antigen retrieval methods (e.g., heat-induced epitope retrieval) and optimize primary antibody dilution based on tissue type. Nuclear and cytoplasmic staining patterns may be observed.
• For immunofluorescence/immunocytochemistry (IF/ICC), fix cells with paraformaldehyde and permeabilize with Triton X-100 to detect nuclear and vesicular SOCS1 localization; include isotype controls.
• As a polyclonal antibody, batch-to-batch variability may occur; perform pilot experiments and consider titrating the antibody for each new application or lot.
• Cross-reactivity with mouse and rat SOCS1 has been confirmed; however, always validate species-specific reactivity in your model system.
• When using this antibody for ELISA, pair with an appropriate detection antibody and validate standard curves with recombinant SOCS1 protein.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a comprehensive range of IVD raw materials, including validated antibodies for key signaling targets such as SOCS1. Our portfolio covers optimized polyclonal antibodies, recombinant proteins, and bulk ancillary reagents, streamlining procurement for cytokine signaling studies and immunoassay development. To support your R&D pipelines, we also offer one-stop sourcing for rare-target raw materials and technical consultancy—bridging the gap from concept to clinic.