Anti-SOAT1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P35610

Core Product Specifications and Parameters

Biological Background: SOAT1 Function and Localization

• Catalyzes the formation of fatty acid-cholesterol esters, which are less soluble in membranes than cholesterol, thereby regulating cholesterol homeostasis. Related references: PMID:16154994, PMID:16647063, PMID:32433613
• Plays a role in lipoprotein assembly and dietary cholesterol absorption. Related references: PMID:16154994, PMID:9020103
• Preferentially utilizes oleoyl-CoA ((9Z)-octadecenoyl-CoA) as a substrate, showing higher activity towards an acyl-CoA substrate with a double bond at the delta-9 position than towards saturated acyl-CoA or unsaturated acyl-CoA with a double bond at other positions. Related references: PMID:11294643, PMID:32433614
• Localizes to the endoplasmic reticulum membrane, the primary site of cellular cholesterol esterification.
• Post-translational modifications include acetylation and phosphorylation; the protein contains disulfide bonds and undergoes alternative splicing, yielding multiple isoforms.
• Functions in cholesterol storage and lipid droplet formation, and is a key enzyme in atherosclerotic foam cell development.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-130), which may detect both full-length and N-terminal isoforms; validate in relevant sample systems.
• For Western blot, expect a major band near 65 kDa; use lysates from human, mouse, or rat tissues/cells.
• In immunofluorescence, anticipate endoplasmic reticulum staining patterns consistent with its subcellular localization.
• For ELISA, consider pairing with a capture antibody or using the antibody itself as capture/detection after thorough optimization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. Our product portfolio supports research in cholesterol metabolism and related disorders. For rare target sourcing or custom antibody development, our technical team offers tailored solutions.