Anti-SOAT1 Monoclonal Antibody for WB, ELISA - P35610

Core Product Specifications and Parameters

Biological Background: SOAT1 Function and Localization

• SOAT1 (sterol O-acyltransferase 1), also known as ACAT1, catalyzes the formation of fatty acid-cholesterol esters, which are less soluble in membranes than free cholesterol, playing a critical role in maintaining cellular cholesterol homeostasis. Related references: PMID:16154994, PMID:16647063, PMID:32433613
• It is involved in lipoprotein assembly and dietary cholesterol absorption, contributing to overall lipid metabolism. Related references: PMID:16154994, PMID:9020103
• The enzyme preferentially uses oleoyl-CoA as a substrate, showing higher activity towards acyl-CoA with a double bond at the delta-9 position (9Z) compared to saturated or other unsaturated acyl-CoAs. Related references: PMID:11294643, PMID:32433614
• SOAT1 localizes to the endoplasmic reticulum membrane, where it functions as a transmembrane protein.
• The protein undergoes post-translational modifications including acetylation and phosphorylation, and contains disulfide bonds.
• Alternative splicing of SOAT1 generates multiple isoforms, which may have distinct functional properties.
• As a key acyltransferase, SOAT1 is a potential therapeutic target for cardiovascular disease and other cholesterol-related disorders.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-104) of human SOAT1, which may be important for antibody specificity; consider validating in your specific experimental system.
• For Western blot, the predicted molecular weight of SOAT1 is approximately 65 kDa; appropriate protein loading and transfer conditions should be empirically determined.
• ELISA applications may require optimization of coating antigen and antibody concentrations; test with recombinant SOAT1 protein or cell lysates.
• Cross-reactivity has been tested with human samples; for other species, sequence homology should be examined before use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to validated IVD raw materials for cholesterol metabolism and cardiovascular disease research. Our offering includes optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and tailored sourcing for rare targets such as SOAT1. Partner with CamelBio for comprehensive support from concept through clinical application.