Anti-SNX9 Polyclonal Antibody for WB and ELISA - Q9Y5X1

Core Product Specifications and Parameters

Biological Background: SNX9 Function and Localization

• Sorting nexin-9, also known as SH3 and PX domain-containing protein 1 (SDP1) and SH3 and PX domain-containing protein 3A.
• Involved in endocytosis and intracellular vesicle trafficking, both during interphase and mitosis. Required for efficient progress through mitosis and cytokinesis, and normal cleavage furrow formation.
• Participates in clathrin-coated pit-mediated endocytosis and clathrin-independent, actin-dependent fluid-phase endocytosis. Also plays a role in macropinocytosis.
• Promotes internalization of TNFR and degradation of EGFR after EGF signaling.
• Stimulates the GTPase activity of DNM1, promotes DNM1 oligomerization, and activates the Arp2/3 complex via WASL, thereby reorganizing the F-actin cytoskeleton.
• Binds to membranes enriched in phosphatidylinositol 4,5-bisphosphate and promotes membrane tubulation; has lower affinity for phosphatidylinositol 3-phosphate-enriched membranes.
• Located at cytoplasmic vesicle membranes, cell membrane, clathrin-coated vesicles, trans-Golgi network, cell ruffles, and cytoplasm.
• Widely expressed, with highest levels in heart and placenta, and lowest in thymus and peripheral blood leukocytes.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-210) of human SNX9, which may include portions of the SH3 and PX domains, potentially contributing to antibody specificity in polyclonal preparations.
• When performing Western blot, a band around 67 kDa is expected; validate in relevant sample systems such as heart or placenta lysates, where SNX9 expression is high.
• For ELISA, consider titrating the antibody to determine the optimal working concentration for your specific assay format.
• Cross-reactivity with mouse and rat SNX9 allows translational studies, but always verify reactivity in your experimental model.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports your SNX9-related research and diagnostic development by providing this high-quality polyclonal antibody, backed by our comprehensive one-stop raw-material sourcing services. From validated antibody pairs to optimized polyclonal and monoclonal reagents, we connect you with the critical tools needed for studying endocytosis, membrane trafficking, and cell division. Explore our extensive catalog and custom sourcing capabilities to accelerate your projects from concept to clinic.