Anti-SNX5 Rabbit Polyclonal Antibody for WB, ELISA - Q9Y5X3

Core Product Specifications and Parameters

Biological Background: SNX5 Function and Localization

• SNX5 is a sorting nexin that selectively binds phosphatidylinositol 3-phosphate and phosphatidylinositol 3,4-bisphosphate, facilitating its recruitment to endosomal membranes.
  Related references:
  PMID:15561769

• As a component of the retromer SNX-BAR subcomplex, SNX5 mediates retrograde transport of cargo proteins (e.g., IGF2R) from endosomes to the trans-Golgi network and deforms donor membranes into tubular endosome-to-TGN transport carriers.
  Related references:
  PMID:17148574, PMID:18596235

• SNX5 is involved in EGFR internalization, endosomal sorting, and lysosomal degradation via a retromer-independent pathway that requires PIP5K1C isoform 3.
  Related references:
  PMID:23602387

• It regulates E-cadherin degradation by inhibiting PIP5K1C isoform 3-mediated sorting.
  Related references:
  PMID:24610942

• SNX5 localizes to early endosomes, cell membrane, cytoplasmic vesicles, and cell projections such as phagocytic cups and ruffles.

• The protein also contributes to macropinocytosis.
  Related references:
  PMID:18854019, PMID:21048941

• SNX5 undergoes post-translational modifications including acetylation and phosphorylation, and is implicated in host-virus interactions and alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 145–404 of human SNX5, covering central and C-terminal regions; ensure your target epitope is present in the sample of interest.
• For Western blotting, expect a band near 47 kDa; use appropriate molecular weight markers and validate in the relevant species (human, mouse, rat) with positive/negative controls.
• For ELISA applications, consider optimizing coating antigen concentration and using recombinant SNX5 protein as a reference standard.
• Cross-reactivity with mouse and rat indicates conserved epitopes across these species; perform sequence alignment to confirm suitability for your model organism.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop supplier of IVD raw materials, offering validated antibodies, bulk ancillary reagents, and rare target sourcing for diagnostic development. This anti-SNX5 polyclonal antibody supports research into retromer-mediated trafficking, EGFR signaling, and endosomal sorting pathways. Contact us for customized antibody pairs, optimized monoclonal antibodies, and technical support to advance your IVD projects.