Anti-SNX32 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q86XE0

Core Product Specifications and Parameters

Biological Background: SNX32 Function and Localization

• Sorting nexin-32 (SNX32), also known as sorting nexin-6B (SNX6B), is a member of the sorting nexin family that facilitates endosomal cargo sorting and trafficking.
• SNX32 regulates endosomal trafficking of key receptors, including transferrin receptor (TFRC), cation-independent mannose-6-phosphate receptor (IGF2R), and epidermal growth factor receptor (EGFR). Related references: PMID:37158588, PMID:39073202
• SNX32 functions redundantly with other BAR domain-containing sorting nexins in endosome-to-trans-Golgi network (TGN) retrieval of IGF2R. Related references: PMID:39073202
• In coordination with SNX5, SNX32 sorts EGF-activated EGFR into multivesicular bodies for lysosomal degradation, modulating EGFR signaling. Related references: PMID:39073202
• SNX32 plays an essential role in neurite outgrowth by controlling the trafficking of basigin (BSG/EMMPRIN) to the cell surface (by similarity).
• Subcellularly, SNX32 localizes to multiple membrane compartments, including the cell membrane, early endosomes, recycling endosomes, late endosomes, and the trans-Golgi network.
• The protein features a coiled-coil domain, alternative splicing, and a partially resolved 3D structure, indicative of its role in membrane remodeling.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a central region (aa 218–285) of human SNX32. When designing similar antibodies, align homologous sequences from mouse and rat to confirm cross-reactivity potential.
• For Western blotting (WB), expect a band near 46 kDa. Validate specificity with positive controls (e.g., HeLa, HEK293) and siRNA knockdown or knockout experiments.
• For immunofluorescence/immunocytochemistry (IF/ICC), note the protein’s endosomal and Golgi localization; use co-localization markers such as EEA1 (early endosome) or TGN46 (trans-Golgi) to confirm.
• ELISA can be used for quantitation; consider using recombinant SNX32 protein as a standard.
• Because SNX32 dynamically localizes to multiple compartments, treatment conditions (e.g., EGF stimulation) may alter staining patterns. Pre-experimental optimization is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, providing comprehensive supply solutions from discovery to clinical application. For neuroscience and cell biology research, we offer a portfolio of validated antibodies, antibody pairs, and ancillary reagents, including this anti-SNX32 polyclonal antibody. Whether you need bulk quantities, custom development, or rare target sourcing, CamelBio streamlines your procurement process with reliable quality and technical support.