Anti-SNX3 Rabbit Polyclonal Antibody for WB and ELISA - O60493

Core Product Specifications and Parameters

Biological Background: SNX3 Function and Localization

• SNX3, also known as Sorting nexin-3 or Protein SDP3, is a phosphoinositide-binding protein encoded by the SNX3 gene.
• It specifically binds phosphatidylinositol 3-phosphate (PtdIns(3)P) and can also interact with PtdIns(4)P, PtdIns(5)P, and PtdIns(3,5)P2, playing a key role in multivesicular body formation.
• In endosomal sorting, SNX3 functions as part of the SNX3-retromer complex to mediate retrograde transport of WLS from endosomes to the trans-Golgi network (TGN), distinct from the SNX-BAR retromer pathway. Related references: PMID:21725319, PMID:24344282, PMID:30213940
• It regulates phagocytosis in dendritic cells by controlling the recruitment of EEA1 to nascent phagosomes. Related references: PMID:23237080
• SNX3 is involved in iron homeostasis by regulating endocytic recycling of the transferrin receptor TFRC, directing the transferrin-TFRC complex to recycling endosomes.
• The protein promotes stability and cell surface expression of epithelial sodium channel (ENaC) subunits and contributes to neurite outgrowth in primary neurons.
• During Salmonella enterica infection, SNX3 promotes maturation of the Salmonella-containing vacuole and recruitment of LAMP1.
• Subcellular localization includes early endosomes, cytoplasmic vesicles, and phagosomes; post-translational modifications include acetylation, methylation, and phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal 162 amino acids, including the PX domain responsible for phosphoinositide binding; this region is well conserved across human, mouse, and rat.
• For Western blotting, the predicted molecular weight is ~19 kDa; consider that post-translational modifications or isoforms may cause slight shifts. Validate in relevant cell or tissue lysates.
• The polyclonal nature may provide sensitive detection in ELISA; for sandwich ELISA, pairing with a monoclonal capture antibody targeting a non-overlapping epitope is recommended.
• Cross-reactivity with mouse and rat has been confirmed by the supplier, making it suitable for rodent model studies. Always include appropriate controls to verify specificity in your system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive supply channel for IVD raw materials, including validated antibody pairs, recombinant proteins, and ancillary reagents for research on endosomal trafficking and retromer biology. This anti-SNX3 rabbit polyclonal antibody exemplifies our commitment to supporting diagnostic manufacturers and researchers with consistent quality and technical support. For bulk orders or customized sourcing solutions, please contact our team to streamline your procurement process.