Anti-SNX15 Polyclonal Antibody for WB, IF/ICC, ELISA - Q9NRS6

Core Product Specifications and Parameters

Biological Background: SNX15 Function and Localization

• SNX15 is a member of the sorting nexin family, characterized by a phox (PX) domain that binds phosphoinositides, enabling membrane association.
• The protein is implicated in intracellular trafficking, especially within the endosomal recycling pathway; overexpression disrupts the normal flow of proteins from the plasma membrane to recycling endosomes or the trans-Golgi network (TGN).
• SNX15 localizes to the cytoplasm, various membranes, and the cytoplasmic face of vesicle membranes, consistent with its dynamic role in vesicle transport.
• Alternative splicing generates multiple transcript variants, and the protein undergoes post-translational modifications such as phosphorylation and methylation, which may regulate its function.
• It is widely expressed across tissues, suggesting a fundamental housekeeping role in cellular transport processes.
• UniProt keywords include “Lipid-binding,” “Protein transport,” and “Reference proteome,” underscoring its established role in endomembrane trafficking and its curated status in proteomics.

Experimental Guidance and Technical Tips

• The immunogen comprises the full-length human SNX15 protein (amino acids 1–342); therefore, the antibody may detect multiple isoforms if the epitopes are shared.
• For Western blotting, prepare lysates with protease and phosphatase inhibitors to preserve phosphorylation and other PTMs, and consider using fresh samples.
• In immunofluorescence or ICC, include permeabilization steps and appropriate subcellular markers to verify membrane/vesicle localization; validate the staining pattern in a biologically relevant cell line or tissue.
• Cross-reactivity with mouse and rat has been observed; for other species, perform sequence alignment and pilot testing to confirm reactivity.
• ELISA can be employed, but optimal coating antigen concentrations and detection conditions should be determined empirically for each assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop sourcing partner for IVD raw materials, supporting diagnostic developers and research institutions from concept to clinic. For intracellular trafficking targets like SNX15, we offer a full spectrum of high-quality raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, recombinant proteins, and bulk ancillary reagents. Our extensive sourcing network and technical expertise help accelerate your projects with reliable, scalable supply solutions.