Anti-SNX1 Rabbit pAb for WB - Q13596

Core Product Specifications and Parameters

Biological Background: SNX1 Function and Localization

• SNX1 is a core component of the retromer SNX-BAR subcomplex, which deforms endosomal membranes into tubules for retrograde transport of cargo proteins from endosomes to the trans-Golgi network. It directly interacts with phosphatidylinositol 3-phosphate (PtdIns(3P)) and PtdIns(3,5)P2 on membranes.
  Related references: PMID:12198132, PMID:19816406, PMID:23085988

• SNX1 targets ligand-activated epidermal growth factor receptor (EGFR) to lysosomes for degradation, ensuring proper signal attenuation after endocytosis.
  Related references: PMID:12198132, PMID:15498486, PMID:17101778

• Beyond retromer, SNX1 participates in retromer-independent endocytic trafficking and lysosomal degradation of receptors such as P2RY1 and protease-activated receptor-1 (PAR1/F2R).
  Related references: PMID:16407403, PMID:20070609

• SNX1 promotes KALRN- and RHOG-dependent membrane remodeling, including lamellipodium formation, independent of the retromer complex.
  Related references: PMID:20604901

• Agonist-induced endocytosis of dopamine receptor DRD5 requires SNX1, while basal receptor trafficking does not.
  Related references: PMID:23152498

• Subcellularly, SNX1 localizes to endosome membranes, the trans-Golgi network, early endosomes, and lamellipodia, consistent with its multifaceted trafficking roles.

• Post-translational modifications include acetylation and phosphorylation, and the gene undergoes alternative splicing, generating multiple isoforms.

Experimental Guidance and Technical Tips

• For Western blot, expect a band near 59 kDa; consider using positive controls such as HeLa or HEK293 cell lysates known to express SNX1.
• In immunofluorescence, anticipate punctate cytoplasmic and perinuclear staining patterns corresponding to endosomes and the trans-Golgi network.
• The polyclonal nature may provide robust signal but validate the antibody in your specific sample system and optimize dilutions for your application.
• Since the immunogen covers the N-terminal 150 amino acids, the antibody may recognize multiple isoforms containing this region.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, offering high-quality antibodies like this anti-SNX1 polyclonal antibody to advance endosomal trafficking research. Our comprehensive portfolio includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing, supporting diagnostic manufacturers and laboratories from concept to clinic.