Anti-SNX1 Rabbit Monoclonal Antibody for WB, ELISA - Q13596

Core Product Specifications and Parameters

Biological Background: Sorting nexin-1 Function and Localization

• Sorting nexin-1 (SNX1, also known as VPS5/HsT17379) is a component of the retromer complex, playing a critical role in endosome-to-trans-Golgi network (TGN) retrograde transport.
• SNX1 binds phosphatidylinositol 3-phosphate (PtdIns(3P)) and phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2) to associate with endosomal membranes. Related references: PMID:12198132
• As part of the SNX-BAR subcomplex, it deforms membranes into tubular profiles for cargo trafficking; it can sense membrane curvature and has in vitro vesicle-to-membrane remodeling activity. Related references: PMID:19816406, PMID:23085988
• SNX1 mediates retrograde transport of lysosomal enzyme receptors (IGF2R, M6PR, SORT1) and bacterial toxin stxB, and targets ligand-activated EGFR to lysosomes for degradation. Related references: PMID:12198132, PMID:15498486, PMID:17101778
• It also participates in retromer-independent trafficking, such as endocytosis of P2RY1 and lysosomal degradation of protease-activated receptor-1/F2R. Related references: PMID:16407403, PMID:20070609
• SNX1 promotes KALRN- and RHOG-dependent membrane remodeling for lamellipodium formation, independent of retromer. Related references: PMID:20604901
• SNX1 localizes to endosome membranes, trans-Golgi network, early endosomes, and lamellipodia, consistent with its roles in trafficking and membrane dynamics.
• The protein undergoes post-translational modifications including acetylation and phosphorylation, and alternative splicing generates multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-100) of human SNX1; consider this when designing detection or blocking strategies.
• For Western blot, the predicted molecular weight is ~59kDa; verify band specificity using appropriate controls and account for potential isoforms or post-translational modifications.
• ELISA applications may require optimization of coating conditions and antibody titration; cross-reactivity has been demonstrated for human SNX1, so testing in relevant species is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents for endocytosis and vesicle trafficking targets like SNX1. Our comprehensive portfolio supports the development of assays for studying retromer-mediated sorting and lysosomal degradation pathways. Benefit from streamlined procurement of rare target reagents and technical consultation to accelerate your project from concept to clinic.