Anti-SNW1 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q13573

Core Product Specifications and Parameters

Biological Background: SNW1 Function and Localization

• SNW1 (SNW domain-containing protein 1), also known as Ski-interacting protein (SKIP), Nuclear receptor coactivator NCoA-62, and Bx42, is encoded by the SNW1 gene. It is a multifunctional nuclear protein involved in pre-mRNA splicing and transcriptional regulation.
• As a core component of the spliceosome, SNW1 participates in the splicing of U12-type introns (minor spliceosome) and is required for the specific splicing of CDKN1A pre-mRNA, likely by recruiting U2AF2. It may also recruit PPIL1 to the spliceosome and contributes to cyclin-D1 (CCND1) mRNA stability via the SNARP complex. Related references: PMID:11991638, PMID:28076346, PMID:28502770
• SNW1 modulates transcriptional activity through interactions with numerous factors: it mediates TGF-β signaling via SMAD proteins, MYOD1-dependent transcription via PABPN1, RB1-mediated repression, and RXR/VDR-dependent gene transcription involving coactivators NCOA1 and GRIP1. It also acts as a coactivator for NOTCH1 by binding to multimerized NICD and recruiting MAML1.
• The protein localizes exclusively to the nucleus, consistent with its roles in mRNA processing and transcription.
• Post-translational modifications include phosphorylation, acetylation, isopeptide bond formation, and ubiquitin-like conjugation, which may regulate its activity and interactions.
• During viral infection, SNW1 is hijacked by HIV-1 Tat to enhance viral transcription by recruiting MYC, MEN1, and TRRAP to the HIV promoter. It is also implicated in transcriptional activation by EBV EBNA2.
• Keywords: mRNA processing, mRNA splicing, transcription, transcription regulation, host-virus interaction.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 300–536 of human SNW1, a region that may include functional domains; verify sequence conservation if working with non-human species.
• Validated applications are WB, IHC-P, IF/ICC, and ELISA. For western blot, consider a starting dilution in the range of 1:500–1:2000, optimized for your sample type.
• For IHC-P and IF/ICC, antigen retrieval (e.g., citrate buffer, pH 6.0) and permeabilization (e.g., 0.1% Triton X-100) are typically required. Nuclear staining should be expected.
• Confirm suitability in your experimental system, as performance can vary with sample preparation and detection reagents. The antibody cross-reacts with mouse and rat, but endogenous expression levels should be validated.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This SNW1 antibody supports research in transcription, splicing, and host-pathogen interactions. We offer validated polyclonal and monoclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets, ensuring your projects progress efficiently.