Anti-SNRPF Polyclonal Antibody for WB, IHC-P, ELISA - P62306

Core Product Specifications and Parameters

Biological Background: Small Nuclear Ribonucleoprotein F (SNRPF) Function and Localization

• SNRPF, also known as Sm protein F (Sm-F), is a core member of the Sm protein family that assembles into spliceosomal small nuclear ribonucleoproteins (snRNPs).
• It plays a critical role in pre-mRNA splicing as a component of the U1, U2, U4, and U5 snRNPs, which are the building blocks of the major spliceosome. Related references: PMID:11991638, PMID:18984161, PMID:19325628
• SNRPF is found in both the pre-catalytic spliceosome B complex and the catalytically activated C complex, reflecting its dynamic participation in splicing catalysis. Related references: PMID:11991638, PMID:28076346, PMID:28502770
• It also functions in the minor spliceosome, where it mediates the splicing of rare U12-type introns. Related references: PMID:15146077
• In addition, SNRPF participates in histone pre-mRNA 3′-end processing as a subunit of the U7 snRNP. Related references: PMID:12975319
• The protein localizes to both the cytoplasm and nucleus, consistent with its roles in snRNP assembly and splicing.
• Post-translational modifications include acetylation, and the protein is classified as an RNA-binding ribonucleoprotein.

Experimental Guidance and Technical Tips

• For Western blot (WB), a band at approximately 10 kDa is anticipated; due to the small size, use a 15% or gradient gel and appropriate transfer conditions for low-molecular-weight proteins.
• In immunohistochemistry (IHC-P), nuclear staining is expected given SNRPF's subcellular localization; optimize antigen retrieval (e.g., citrate buffer, pH 6.0) and include nuclear markers for co-localization validation.
• The immunogen corresponds to the full-length protein (a.a. 1-86), which may detect both assembled and unassembled forms; consider cross-linking or native gel analyses for snRNP complexes.
• Cross-reactivity with mouse and rat SNRPF is predicted based on sequence homology; however, verify reactivity in the relevant species and tissue type via positive and negative controls.
• For ELISA applications, use recombinant SNRPF or nuclear extract as the coating antigen; assess lot-to-lot consistency for quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement by offering validated antibodies, optimized monoclonals, and bulk ancillary reagents for splicing and snRNP research. With a focus on rare targets like SNRPF, we help diagnostic manufacturers and labs accelerate assay development from concept to clinic. Explore our portfolio for reliable polyclonal and monoclonal antibodies, recombinant proteins, and custom sourcing solutions.