Anti-SNRPE Polyclonal Antibody for WB, IHC-P, ELISA - P62304

Core Product Specifications and Parameters

Biological Background: SNRPE Function and Localization

• SNRPE encodes Sm protein E (Sm-E), a core component of small nuclear ribonucleoproteins (snRNPs).
• As part of the major spliceosome, it is essential for pre-mRNA splicing and participates in the formation of U1, U2, U4, and U5 snRNPs. Related references: PMID:11991638 PMID:18984161 PMID:19325628
• It is present in both the pre-catalytic spliceosome B complex and the activated spliceosome C complex. Related references: PMID:11991638 PMID:28076346 PMID:28502770
• In the minor spliceosome, SNRPE contributes to the splicing of U12-type introns. Related references: PMID:15146077
• As a component of the U7 snRNP, it plays a role in histone 3'-end processing. Related references: PMID:12975319
• SNRPE localizes to the cytoplasm, cytosol, and nucleus.
• Widely expressed, with presence in scalp skin including the hair follicle, epidermis, and dermis.
• Mutations in SNRPE are associated with hypotrichosis (hair loss disorders).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal 92 amino acids of human SNRPE, covering nearly the entire protein. This design may be suitable for detecting endogenous SNRPE in various applications.
• For Western blot (WB), consider that the predicted molecular weight is approximately 11 kDa. Validate the antibody in your specific sample system and optimize conditions.
• For immunohistochemistry (IHC-P), antigen retrieval protocols and dilution factors should be empirically determined. Use appropriate controls.
• For ELISA, note that the antibody is a polyclonal preparation; consider pair testing for sandwich assays if needed.
• The antibody cross-reacts with mouse and rat SNRPE, enabling multi-species studies. However, always verify cross-reactivity in your model organism.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with seamless access to IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Our focus on spliceosome and RNA processing targets supports your assay development from concept to clinic, with rare target sourcing and technical services available upon request.