Anti-SNRPD3 Rabbit Polyclonal Antibody for WB, ELISA - P62318

Core Product Specifications and Parameters

Biological Background: SNRPD3 Function and Localization

• SNRPD3 encodes Sm-D3, a core protein component of the U1, U2, U4, and U5 small nuclear ribonucleoproteins (snRNPs), which are essential building blocks of the major spliceosome.
  Related references: PMID:11991638, PMID:18984161, PMID:19325628
• It is present in both the pre-catalytic spliceosome B complex and the activated C complex, participating directly in the catalytic steps of pre-mRNA splicing.
  Related references: PMID:11991638, PMID:28076346, PMID:28502770
• Beyond the major spliceosome, SNRPD3 is also a component of the minor spliceosome, where it mediates the splicing of rare U12-type introns found in a subset of human pre-mRNAs.
  Related references: PMID:15146077, PMID:33509932
• As part of the U7 snRNP, Sm-D3 participates in the 3′-end processing of histone pre-mRNAs, linking it to cell-cycle-dependent histone biosynthesis (by similarity).
• The protein localizes dynamically between the cytoplasm, cytosol, and nucleus, consistent with its assembly and recycling in snRNP biogenesis and splicing.
• Post-translational modifications include acetylation and methylation, and the gene undergoes alternative splicing, contributing to functional diversity and regulation.
• The antibody’s cross-reactivity with human, mouse, and rat reflects the high sequence conservation of SNRPD3 across mammalian species.

Experimental Guidance and Technical Tips

• For Western blot detection, a band at approximately 14 kDa is expected in lysates from human, mouse, or rat cells. Confirm specificity and absence of cross-reactivity in your particular sample matrix.
• Because the immunogen spans the N‑terminal region (aa 8–121) of SNRPD3, the antibody may recognize both full-length and truncated isoforms; validate in your model system if isoform discrimination is critical.
• In ELISA applications, use the recombinant immunogen as a positive control and titrate the antibody to optimize the signal-to-noise ratio.
• As a polyclonal antibody, lot‑to‑lot consistency should be verified; consider running a reference sample with each new lot when establishing long‑term protocols.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw material procurement for manufacturers and laboratories developing splicing-targeted diagnostics. This anti-SNRPD3 polyclonal antibody is part of a broader portfolio that includes validated antibody pairs, monoclonal antibodies, and bulk ancillary reagents covering spliceosome and mRNA processing targets. Our sourcing network ensures timely access to rare-target reagents, supporting your assay development from concept to clinical validation.