Anti-SNRPD1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P62314

Core Product Specifications and Parameters

Biological Background: SNRPD1 Function and Localization

• SNRPD1 encodes the small nuclear ribonucleoprotein Sm D1, a core component of the Sm protein family forming the structural scaffold of spliceosomal snRNPs.
• SNRPD1 is a core component of the spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), essential for pre-mRNA splicing. Related references: PMID:11991638, PMID:18984161, PMID:19325628
• The protein is incorporated into both pre-catalytic B complex and activated C complex spliceosomes, mediating successive steps of the splicing reaction. Related references: PMID:11991638, PMID:26912367, PMID:28076346
• SNRPD1 also functions in the minor spliceosome, facilitating splicing of rare U12-type introns. Related references: PMID:15146077
• The protein may serve as an electrostatic RNA scaffold, promoting snRNP assembly and inter-snRNP contacts via non-specific interactions. Related references: PMID:23333303
• SNRPD1 localizes to both the nucleus and the cytoplasm (including the cytosol), reflecting its roles in snRNP biogenesis and recycling.
• Post-translational modifications include methylation and isopeptide bond formation (Ubl conjugation), which are critical for snRNP assembly and regulatory control.

Experimental Guidance and Technical Tips

• The immunogen comprises the N-terminal 96 amino acids of SNRPD1; consider this region’s conservation when assessing cross-reactivity.
• For Western blot, the predicted molecular weight is ~13 kDa; optimize gel percentage and transfer conditions for small proteins.
• In IHC-P and IF/ICC, validate nuclear and cytoplasmic localization patterns in relevant tissue or cell lines.
• ELISA performance may depend on antigen coating conditions; titrate antibody concentration for optimal signal.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive IVD raw-material sourcing solution, providing high-quality antibodies such as this anti-SNRPD1 polyclonal antibody for spliceosome and mRNA processing research. Our platform supplies validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing, helping diagnostic manufacturers and labs streamline their development pipelines from early-stage research to clinical application.