Anti-SNRPB2 Rabbit Polyclonal Antibody for WB, ELISA - P08579

Core Product Specifications and Parameters

Biological Background: SNRPB2 Function and Localization

• SNRPB2 encodes the U2 small nuclear ribonucleoprotein B'' (also known as Msl1 or U2B''), a core component of the U2 snRNP complex essential for pre-mRNA splicing.
• It functions as part of the spliceosome, catalyzing the removal of introns from pre-mRNA transcripts, a critical step in gene expression.
  Related references: PMID:11991638, PMID:28076346, PMID:28502770
• Within U2 snRNP, SNRPB2 specifically binds to stem-loop IV of the U2 small nuclear RNA (snRNA), stabilizing the complex and ensuring accurate splice site recognition.
  Related references: PMID:32494006, PMID:9716128
• The protein is predominantly localized to the nucleus, consistent with its role in splicing and association with nuclear ribonucleoprotein complexes.
• SNRPB2 is subject to post-translational modifications including phosphorylation and acetylation, which may modulate its activity or interactions within the spliceosome. Additional structural features include isopeptide bonds and ubiquitin conjugation.
• It belongs to a family of spliceosomal RNA-binding proteins and contains conserved RNA recognition motifs, reflected in keywords such as mRNA processing, spliceosome, and ribonucleoprotein.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–150) of human SNRPB2, a domain conserved across human, mouse, and rat, supporting the observed cross-reactivity.
• For Western blot, the expected band size is approximately 25 kDa; consider using a positive control such as HeLa or HEK293 nuclear extract to confirm specificity.
• In ELISA applications, optimize coating antigen and antibody dilutions; the polyclonal nature may require careful titration to minimize background.
• Potential variations in band mobility due to post-translational modifications should be considered; validate by phosphatase treatment or overexpression/knockdown systems.
• Although not yet validated for other applications, this antibody may be suitable for immunoprecipitation or immunofluorescence given its nuclear target; pilot experiments are recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories by providing a comprehensive portfolio of IVD raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, and ancillary reagents. For projects targeting splicing factors like SNRPB2, we offer customized sourcing solutions for rare targets and bulk supply of optimized antibodies to accelerate assay development from concept to clinic. Partner with CamelBio for reliable, end-to-end procurement support in the molecular diagnostics field.