Anti-SNRPB Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P14678

Core Product Specifications and Parameters

Biological Background: SNRPB Function and Localization

• SNRPB encodes Sm proteins B and B' (Sm-B/B'), core components of the spliceosomal U1, U2, U4, and U5 snRNPs, essential for pre-mRNA splicing. Related references: PMID:11991638, PMID:18984161, PMID:19325628
• It is a component of both the pre-catalytic spliceosome B complex and the activated C complex, indicating roles throughout the splicing cycle. Related references: PMID:11991638, PMID:28076346, PMID:28502770
• As part of the minor spliceosome, SNRPB is involved in splicing U12-type introns, highlighting its function in alternative splicing pathways. Related references: PMID:15146077
• SNRPB also participates in histone pre-mRNA 3'-end processing as a component of the U7 snRNP. Related references: PMID:12975319
• The protein localizes to the nucleus, cytosol, and cytoplasm, reflecting its dynamic roles in RNA processing.
• Post-translational modifications include methylation, and alternative splicing produces the B and B' isoforms; mutations in SNRPB are linked to intellectual disability disorders.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa1–100) of human SNRPB, which may be conserved between the B and B' isoforms; verify isoform recognition if differential detection is required.
• For Western blot, expect a band around 25 kDa; validate in cell or tissue lysates from human, mouse, or rat, noting that expression levels can vary across cell types.
• For immunohistochemistry (IHC-P), optimize antigen retrieval and antibody dilution experimentally; anticipate nuclear and cytoplasmic staining consistent with localization data.
• For ELISA, the peptide immunogen supports direct ELISA for titering; consider using appropriate controls when developing sandwich or competitive ELISA formats.
• As a polyclonal antibody, it may exhibit lot-to-lot variation; include positive and negative controls in each experiment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive one-stop sourcing solution for IVD raw materials, pairing this anti-SNRPB antibody with validated antigen pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. Supporting splicing and RNA processing research, we help diagnostic manufacturers and labs secure rare target reagents and technical services from concept through clinical translation.