Anti-SNRNP70 Rabbit Polyclonal Antibody for WB, ELISA - P08621

Core Product Specifications and Parameters

Biological Background: SNRNP70 Function and Localization

• SNRNP70 is a core component of the U1 small nuclear ribonucleoprotein (snRNP) complex, which plays a critical role in pre-mRNA splicing by recognizing the 5' splice site and facilitating spliceosome assembly. Related references: PMID:19325628, PMID:25555158
• SNRNP70 specifically binds to the loop I region of U1 snRNA, a key RNA-RNA interaction necessary for splicing. Related references: PMID:19325628, PMID:2467746, PMID:25555158
• Truncated isoforms lacking the RRM domain fail to bind U1 snRNA, highlighting the domain's importance for function.
• Localizes to nuclear speckles and nucleoplasm, consistent with its role in pre-mRNA processing.
• Known to undergo acetylation, phosphorylation, and ubiquitin conjugation, which may regulate its splicing activity.
• Alternative splicing produces multiple isoforms, expanding functional diversity.
• As a ribonucleoprotein involved in mRNA processing, SNRNP70 is essential for accurate gene expression.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (aa 1-180), which includes the RNA recognition motif (RRM) domain; consider this epitope location when designing experiments.
• For Western blot, the predicted molecular weight is approximately 52 kDa; consider using reducing conditions and a suitable loading control.
• Validate antibody specificity in knockout, knockdown, or siRNA experiments to confirm target recognition.
• For ELISA, optimize antibody dilutions empirically; the antibody may serve as a capture or detection reagent.
• Cross-reactivity with mouse and rat suggests epitope conservation across species; sequence alignment may confirm suitability for these models.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a streamlined sourcing solution for IVD raw materials, including this anti-SNRNP70 polyclonal antibody for applications in splicing research and assay development. Our supply network extends to validated antibody pairs, bulk monoclonal/polyclonal antibodies, ancillary reagents, and rare target sourcing, supporting your projects from concept to clinical application.