Anti-SNRNP70 Rabbit Monoclonal Antibody for WB and ELISA - P08621

Core Product Specifications and Parameters

Biological Background: SNRNP70 Function and Localization

• SNRNP70 encodes the U1 small nuclear ribonucleoprotein 70 kDa, a key protein component of the U1 snRNP particle.
• It is essential for pre-mRNA splicing by recognizing the 5' splice site within the spliceosome. Related references: PMID:19325628 PMID:25555158
• SNRNP70 directly binds the loop I region of U1 snRNA through its RNA recognition motif (RRM) domain. Related references: PMID:19325628 PMID:2467746 PMID:25555158
• Truncated isoforms lacking the RRM domain fail to interact with U1 snRNA, indicating isoform-specific functional differences.
• Localizes predominantly to nuclear speckles and the nucleoplasm, consistent with its role in pre-mRNA processing.
• Subject to various post-translational modifications including acetylation, phosphorylation, and ubiquitination, which may regulate its activity.
• Undergoes alternative splicing, generating multiple isoforms with potentially distinct functions.
• Classified as a ribonucleoprotein with RNA-binding capacity, and plays a role in Ubl conjugation pathways.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a peptide within the central region (aa 201-300) of SNRNP70, suggesting the antibody targets a sequence distinct from the N-terminal RRM domain; consider this when interpreting results with isoforms or deletion mutants.
• For western blotting, use whole-cell or nuclear extracts from human or mouse cells; the expected molecular weight is approximately 52 kDa, but additional bands may appear due to post-translational modifications or isoform variants.
• In ELISA applications, the peptide immunogen can serve as a positive control; ensure coating conditions are optimized for peptide antigens.
• The KD validation tag implies that siRNA-mediated knockdown experiments have confirmed specificity; nevertheless, proper controls (e.g., untreated lysates, non-targeting siRNA) should always be included.
• When working with mouse samples, confirm cross-reactivity under your specific conditions, as reactivity may vary by tissue or cell line.
• Store the antibody as recommended (ice bag shipping) and avoid repeated freeze/thaw cycles to maintain activity; for long-term storage, aliquot and keep at -20°C or below.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, supporting developers from early research to clinical applications. The SNRNP70 antibody product facilitates spliceosome-related diagnostics and research; CamelBio also provides a wide portfolio of validated antibody pairs, monoclonal antibodies, and bulk reagents, and can assist in sourcing rare or custom targets to streamline your assay development.