Anti-SNRNP70 Polyclonal Antibody for WB, IHC-P, ELISA - P08621

Core Product Specifications and Parameters

Biological Background: U1 Small Nuclear Ribonucleoprotein 70 kDa Function and Localization

• SNRNP70 encodes the U1 small nuclear ribonucleoprotein 70 kDa, a core component of the U1 snRNP particle. Alternative splicing produces truncated isoforms lacking the N-terminal RNA recognition motif (RRM).
• As part of the spliceosomal U1 snRNP, SNRNP70 is essential for recognition of the pre-mRNA 5' splice-site and assembly of the spliceosome.
• The protein directly binds to the loop I region of U1-snRNA via its RRM; truncated isoforms lacking this domain cannot bind U1-snRNA. Related references: PMID:19325628 PMID:25555158 PMID:2467746
• SNRNP70 localizes predominantly to nuclear speckles and the nucleoplasm, consistent with its role in pre-mRNA processing.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitination, which may regulate its activity and interactions.
• The protein is classified under ribonucleoprotein, RNA-binding, and mRNA processing categories, highlighting its critical role in gene expression.

Experimental Guidance and Technical Tips

• The immunogen sequence (aa 150–250) lies outside the canonical RNA recognition motif, potentially enabling recognition of both full‑length and some truncated isoforms.
• For western blot, expect a band around 52 kDa; validate detection in a relevant cell or tissue system expressing SNRNP70.
• For immunohistochemistry (IHC‑P), nuclear staining is anticipated; optimize antigen retrieval and antibody dilution using known positive tissues.
• In ELISA, the synthetic peptide may serve as a capture antigen; consider testing direct vs. sandwich formats and cross‑reactivity with homologous proteins.
• As the product is a polyclonal antibody, lot‑to‑lot consistency should be monitored in critical quantitative assays.

CamelBio: Your One‑Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with a single access point for IVD raw materials, from validated antibody pairs and optimized polyclonal antibodies to bulk ancillary reagents. Our portfolio supports spliceosome and RNA‑processing targets like SNRNP70, helping you secure consistent supply for assay development. With end‑to‑end technical and sourcing support, CamelBio bridges the gap from concept to clinic.