Anti-SNRNP35 Polyclonal Antibody for WB, ELISA - Q16560

Core Product Specifications and Parameters

Biological Background: SNRNP35 Function and Localization

• SNRNP35, also known as U11/U12 small nuclear ribonucleoprotein 35 kDa protein, HM-1, and U1 snRNP-binding protein homolog, is a component of the minor (U12-dependent) spliceosome.
• It participates in pre-mRNA splicing by associating with U11 and U12 snRNPs, playing a role in the recognition and removal of the minor class of introns.
• The protein is encoded by the SNRNP35 gene (GeneID: 11066) in humans.
• SNRNP35 is localized to the nucleus, consistent with its role in RNA processing.
• Tissue expression is observed in heart, liver, skeletal muscle, and pancreas.
• Post-translational modifications include isopeptide bond formation and ubiquitin-like conjugation (Ubl conjugation), which may regulate its activity or interactions.
• The protein belongs to the mRNA processing and spliceosome pathways, and is classified under RNA-binding proteins.

Experimental Guidance and Technical Tips

• This polyclonal antibody was generated using an N-terminal immunogen (aa 1-150), which may detect full-length SNRNP35 as well as potential N-terminal fragments or isoforms arising from alternative splicing.
• In Western blot, a band around 29 kDa is expected for endogenous SNRNP35; however, validate specificity in your sample system using positive and negative controls.
• For ELISA, optimize coating antigen concentration and antibody dilution to achieve optimal signal-to-noise ratio.
• Considering the nuclear localization, appropriate lysis and fractionation protocols may be needed to enrich the target protein from cell or tissue extracts.
• The antibody cross-reacts with human and mouse, allowing versatility in model systems, but confirm cross-reactivity in your specific application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with comprehensive access to IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies like this anti-SNRNP35, and bulk ancillary reagents. We support projects targeting spliceosome and RNA processing research, offering one-stop sourcing from concept to clinic. Our supply capabilities cover rare and custom targets, ensuring continuity and scalability for your development needs.