Anti-SNRNP200 Rabbit Polyclonal Antibody for WB, ELISA - O75643

Core Product Specifications and Parameters

Biological Background: SNRNP200 Function and Localization

• SNRNP200 (also designated BRR2, ASCC3L1, U5-200KD) is an ATP-dependent RNA helicase that serves as a core component of the U5 small nuclear ribonucleoprotein (snRNP) and the spliceosome.
• It catalyzes the ATP-dependent unwinding of U4/U6 RNA duplexes, an essential step for assembly of a catalytically active spliceosome. Related references: PMID:35241646
• The protein participates in precatalytic, catalytic, and postcatalytic spliceosomal complexes, mediating dynamic RNA-RNA interactions during pre-mRNA splicing. Related references: PMID:28502770 PMID:28781166 PMID:29301961
• As part of the minor spliceosome, SNRNP200 is involved in splicing U12-type introns (Probable).
• Subcellular localization is predominantly nuclear, consistent with its role in splicing.
• The gene is widely expressed across human tissues.
• Post-translational modifications include acetylation and phosphorylation, and the protein contains ATP-binding and helicase domains.
• Mutations in SNRNP200 are linked to retinitis pigmentosa, underscoring its clinical relevance.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1947–2136 of human SNRNP200; consider that the resulting polyclonal antibody may predominantly recognize epitopes within this C-terminal region.
• For Western blot, due to the large molecular weight (245 kDa), run samples on a low-percentage SDS-PAGE gel (e.g., 6–8%) and extend transfer time to ensure efficient blotting.
• In ELISA applications, validate the antibody with recombinant SNRNP200 protein or specific peptides to assess reactivity and specificity.
• Always include appropriate positive and negative controls from human cell lines expressing SNRNP200.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop IVD raw-material sourcing partner offering validated antibodies against spliceosomal targets like SNRNP200. Our supply capabilities include optimized polyclonal antibodies, antibody pairs, and bulk ancillary reagents to support your pre-mRNA splicing research and diagnostic assay development. For rare or custom target sourcing, our technical team assists from concept to clinic.