Anti-SNIP1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q8TAD8

Core Product Specifications and Parameters

Biological Background: SNIP1 Function and Localization

• Encoded by the SNIP1 gene in humans, Smad nuclear-interacting protein 1 (SNIP1) is an FHA domain-containing protein that functions as a component of the spliceosome. Required for pre-mRNA splicing, it is specifically involved in the splicing of U12-type introns via the minor spliceosome. Related references: PMID:29360106
• Down-regulates NF-kappa-B signaling by competing with the RELA subunit for binding to the transcriptional coactivators CREBBP/EP300.
• Contributes to the biogenesis of microRNAs (miRNAs), linking splicing and RNA silencing pathways.
• May regulate cyclin-D1/CCND1 mRNA stability through the SNARP complex, which associates with both the CCND1 gene and its mRNA.
• Localizes predominantly to the nucleus, consistent with its roles in splicing and transcriptional regulation.
• Ubiquitously expressed in human tissues, with highest expression levels observed in heart and skeletal muscle.
• Post-translational modifications include phosphorylation and ubiquitin conjugation (isopeptide bond), as identified by proteomics studies.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a central region of human SNIP1 (aa 200–300). For western blot (WB), expect a band at approximately 46 kDa. Validate in your sample system; cross-reactivity with rat has been reported.
• In IHC-P and IF/ICC, nuclear staining is anticipated. Optimize antigen retrieval and permeabilization conditions for your tissue or cell type.
• For ELISA, consider peptide coating or indirect detection methods to assess antibody reactivity.
• Given SNIP1's involvement in multiple pathways, confirm specificity via knockdown or knockout controls where possible.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, connecting diagnostic developers with verified antibodies and ancillary reagents for splicing and signaling research. Our portfolio features this high-quality anti-SNIP1 rabbit polyclonal antibody—suitable for WB, IHC, IF, and ELISA—backed by reliable supply chains and flexible procurement options. Complement your assay development with our comprehensive offerings, from antigen standards to detection kits, and let us bridge your concept-to-clinic journey.