Anti-SND1 Rabbit Monoclonal Antibody [KO Validated] for WB, IHC-P, IF/ICC, ELISA - Q7KZF4

Core Product Specifications and Parameters

Biological Background: SND1 Function and Localization

• SND1 (Staphylococcal nuclease domain-containing protein 1), also known as p100 co-activator, TDRD11, or Tudor-SN, is a 102 kDa protein that functions as an endonuclease mediating miRNA decay, regulating both protein-free and AGO2-loaded miRNAs (PubMed:18453631, PubMed:28546213). Related references: PMID:18453631, PMID:28546213
• Through its role in miRNA turnover, SND1 controls the expression of mRNAs involved in G1-to-S phase cell cycle transition (PubMed:28546213). Related references: PMID:28546213
• SND1 acts as a transcriptional coactivator, bridging STAT6 with the basal transcription machinery (PubMed:12234934) and mediating PIM1 regulation of MYB activity (PubMed:9809063). Related references: PMID:12234934, PMID:9809063
• During viral infection, SND1 functions as a coactivator for Epstein-Barr virus nuclear antigen 2 (EBNA2) and promotes SARS-CoV-2 RNA synthesis by interacting with negative-sense viral RNA and nsp9.
• Subcellularly, SND1 localizes to the cytoplasm, nucleus, and melanosomes, reflecting its diverse roles in RNA metabolism and transcriptional regulation.
• SND1 is ubiquitously expressed across tissues and is subject to post-translational modifications including acetylation, phosphorylation, and ubiquitination, which may modulate its activity.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 26-285 of human SND1) spans the N-terminal region containing staphylococcal nuclease domains. For Western blot, anticipate a specific band near 102 kDa; consider using reducing conditions and molecular weight markers.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), validate the antibody in known SND1-positive tissues such as testis or liver. Standard antigen retrieval for FFPE sections is recommended.
• The antibody is reported to cross-react with mouse and rat SND1. Verify reactivity with your specific samples, as sequence homology may influence detection.
• In ELISA and functional assays, this antibody may be useful for studying SND1’s endonuclease activity or protein interactions, but appropriate controls and assay optimization are essential.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a reliable one-stop supplier for diagnostic manufacturers and research laboratories, offering a broad portfolio of validated antibodies and IVD raw materials. This anti-SND1 monoclonal antibody is suitable for research into gene regulation, cell cycle control, and host-virus interactions, including SARS-CoV-2 and EBV. Beyond single reagents, CamelBio supports your projects with customized antibody pairs, bulk ancillary reagents, and expert sourcing for rare targets, enabling streamlined IVD development from concept to clinic.