Anti-SNAPIN Polyclonal Antibody for WB, IHC-P, ELISA - O95295

Core Product Specifications and Parameters

Biological Background: SNAPIN Function and Localization

• SNAPIN is a SNARE-associated protein that functions as a component of the biogenesis of lysosome-related organelles complex 1 (BLOC-1), essential for the normal biogenesis of lysosome-related organelles (LROs) such as platelet dense granules and melanosomes.
• In concert with the AP-3 complex, SNAPIN-containing BLOC-1 targets membrane protein cargos into vesicles for delivery into neurites and nerve terminals, and is involved in neurite extension.
• SNAPIN plays a role in intracellular vesicle trafficking and synaptic vesicle recycling in neuronal cells.
• It modulates a step between vesicle priming, fusion, and calcium-dependent neurotransmitter release through its ability to potentiate the interaction of synaptotagmin with SNARE proteins and SNAP25. Related references: PMID:17182842, PMID:18167355
• As part of the BORC complex, SNAPIN contributes to lysosome movement and localization at the cell periphery by recruiting ARL8B and coupling lysosomes to microtubule plus-end-directed kinesin motors. Related references: PMID:25898167
• SNAPIN is localized to multiple cellular compartments, including the membrane, cytoplasm, Golgi apparatus, lysosome, and synaptic vesicle membranes.
• At the protein level, SNAPIN is expressed in male germ cells of adult testis, suggesting a role in spermatogenesis.
• SNAPIN is subject to post-translational modifications such as acetylation and phosphorylation, which may regulate its function in exocytosis. Mutations in SNAPIN are associated with intellectual disability, underscoring its importance in neuronal function.

Experimental Guidance and Technical Tips

• The immunogen covers the full-length human SNAPIN protein (amino acids 1–136), so the polyclonal antibody is expected to recognize multiple epitopes. For Western blotting, the predicted molecular weight is ~15 kDa; consider using a 12–15% SDS-PAGE gel and optimize transfer conditions for small proteins.
• For immunohistochemistry (IHC-P), consider performing antigen retrieval in citrate buffer (pH 6.0) or Tris-EDTA (pH 9.0), as SNAPIN is a membrane-associated protein. Positive staining may be observed in brain neuronal cells or testis, but validation in your specific tissue samples is recommended.
• The antibody cross-reacts with mouse and rat, allowing for interspecies studies. However, sequence alignment of the immunogen region between species is advisable to confirm epitope conservation.
• As a polyclonal antibody, lot-to-lot variability may occur; for critical quantitative assays like ELISA, titrate each new lot to determine optimal working dilution.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your comprehensive one-stop sourcing partner for IVD raw materials, connecting diagnostic manufacturers and research laboratories with high-quality reagents. This anti-SNAPIN antibody supports research into lysosome biology, vesicle trafficking, and neurobiology. CamelBio’s portfolio includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing, all backed by technical and consulting services from concept to clinic.