Anti-SNAP25 Polyclonal Antibody for WB, IF, IHC, ELISA - P60880

Core Product Specifications and Parameters

Biological Background: SNAP25 Function and Localization

• Synaptosomal-associated protein 25 (SNAP25) is a t-SNARE protein involved in the molecular regulation of neurotransmitter release, playing a critical role in the synaptic function of specific neuronal systems.
• It associates with proteins involved in vesicle docking and membrane fusion, and regulates plasma membrane recycling through its interaction with CENPF.
• In pancreatic beta cells, SNAP25 modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1.
• Subcellularly, SNAP25 localizes to the cytoplasm, perinuclear region, cell membrane, synapse, and synaptosome; it is also found in the photoreceptor inner segment.
• Expression is prominent in neurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum.
• The protein undergoes palmitoylation, phosphorylation, and alternative splicing, and is characterized as a coiled coil, lipoprotein, and membrane-associated protein.
• Clinically, genetic variants in SNAP25 are associated with epilepsy and intellectual disability, as annotated by UniProt keywords.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (amino acids 1-206) of human SNAP25; validate detection in your specific application, especially when probing cleavage products or isoforms.
• For western blotting, SNAP25 migrates at an expected molecular weight of approximately 23 kDa; confirm using appropriate markers and positive controls.
• In immunofluorescence and immunohistochemistry, optimize fixation and permeabilization to access synaptic targets; antigen retrieval may enhance signal in paraffin-embedded tissues.
• When performing ELISA, ensure the recombinant or peptide antigen matches the immunogen sequence to maintain consistent detection.
• Cross-reactivity has been observed with mouse and rat SNAP25; verify performance in your sample system if using these species.

CamelBio: Your One-Stop Sourcing Bridge

At CamelBio, we provide diagnostic manufacturers, labs, and research institutes with one-stop access to high-quality IVD raw materials and technical support. Our SNAP25 polyclonal antibody is part of a comprehensive portfolio for neuroscience research and assay development. Beyond antibodies, we offer validated antibody pairs, bulk ancillary reagents, and rare target sourcing to streamline your project from concept to clinic.