Anti-SNAP23 Polyclonal Antibody for WB, IHC-P, ELISA - O00161

Core Product Specifications and Parameters

Biological Background: SNAP23 Function and Localization

• SNAP23 (synaptosomal-associated protein 23) is a ubiquitously expressed t-SNARE protein, also known as vesicle-membrane fusion protein SNAP-23, and belongs to the SNARE superfamily essential for intracellular membrane fusion. Related references: PMID:10036234, PMID:11354632, PMID:8663154
• As a target-SNARE (t-SNARE) on the plasma membrane, SNAP23 functions in fusion of transport vesicles with the cell membrane, particularly in immune cells, by forming a trans-SNARE complex with other SNAREs such as STX11. Related references: PMID:30811271
• In macrophages, SNAP23 cooperates with STX11 to regulate the stimulus-dependent vesicular transport of TLR4 from recycling endosomes to the plasma membrane, playing a critical role in innate and adaptive immunity. Related references: PMID:30811271
• In skeletal muscle satellite cells, SNAP23, together with VAMP4 and STX11, facilitates CD36 vesicular transport to the plasma membrane, an essential step in muscle regeneration (By similarity).
• SNAP23 localizes to the cell membrane and is highly expressed in the placenta, though it is found in virtually all tissues.
• Post-translational modifications include acetylation, palmitoylation (a lipid modification anchoring it to the membrane), and phosphorylation, which may regulate its activity and interactions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to nearly full-length SNAP23 (aa 1-211), which may provide broad epitope coverage. Consider using positive controls such as placenta lysates due to high expression.
• For WB, a band at approximately 23 kDa is expected; verify in the relevant sample system and adjust blocking/detection conditions as needed.
• Cross-reactivity with mouse and rat has been reported, but sequence alignment is recommended when extending to other species or specific isoforms.
• IHC-P may require antigen retrieval; test on known positive tissues (e.g., immune cell-rich samples) and include appropriate controls.
• ELISA performance should be validated using the recombinant immunogen or native protein, with careful optimization of coating and detection parameters.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For research into SNARE-mediated membrane trafficking and immune cell biology, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. Let CamelBio bridge the gap between your project needs and global supply.