Anti-Snail Polyclonal Antibody (SNAI1) for WB, IHC-P, IF/ICC, ELISA - O95863

Core Product Specifications and Parameters

Biological Background: Snail Function and Localization

• SNAI1, also known as protein snail homolog 1, is a zinc finger transcriptional repressor belonging to the Snail family.
• SNAI1 is critically involved in inducing the epithelial-to-mesenchymal transition (EMT), a process essential for embryonic mesoderm formation, growth arrest, cell survival, and migration. Related references: PMID:10655587, PMID:15647282, PMID:20389281
• As a transcriptional repressor, SNAI1 binds to E-boxes in the E-cadherin (CDH1) promoter and recruits the histone demethylase KDM1A, leading to decreased dimethylated H3K4 levels and transcriptional silencing of target genes such as CDH1, CLDN7, and KRT8. Related references: PMID:10655587, PMID:20389281, PMID:20562920
• The N-terminal SNAG domain of SNAI1 competes with histone H3 for binding to the KDM1A/RCOR1 demethylase complex, thereby inhibiting H3K4 demethylation and maintaining gene repression. Related references: PMID:20389281, PMID:21300290, PMID:23721412
• During EMT, SNAI1 cooperates with LOXL2 to negatively regulate pericentromeric heterochromatin transcription, contributing to chromatin reorganization. Related references: PMID:16096638
• SNAI1 recruits LOXL2 to pericentromeric regions, where LOXL2 oxidizes histone H3 and promotes release of the heterochromatin protein CBX5/HP1A, enabling acquisition of mesenchymal traits (By similarity).
• SNAI1 associates with transcription factors EGR1 and SP1 to mediate TPA-induced up-regulation of the CDKN2B promoter, possibly via direct binding to a 5'-TCACA-3' element. Related references: PMID:20121949
• SNAI1 is expressed in a variety of tissues, with highest expression in kidney, and is detected in both mesenchymal and epithelial cell lines.

Experimental Guidance and Technical Tips

• The immunogen used for this antibody corresponds to a synthetic peptide encompassing amino acids 200-267 of human SNAI1; this region may provide reactivity with multiple splice variants or processed forms.
• For Western blot (WB), the predicted molecular weight of SNAI1 is ~29 kDa; consider appropriate gel percentage and transfer conditions, and validate detection in cell lines with known mesenchymal characteristics.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), optimize fixation and antigen retrieval methods as SNAI1 localizes to both nucleus and cytoplasm; cross-reactivity with mouse and rat samples should be verified experimentally.
• For ELISA applications, pair with a suitable capture antibody or use directly for antigen detection, bearing in mind potential batch-to-batch variability inherent to polyclonal antibodies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic and research laboratories with a comprehensive suite of IVD raw materials and services, covering every stage from concept to clinic. For researchers studying epithelial-mesenchymal transition (EMT) and cancer metastasis, we offer validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets like Snail/SNAI1. Our one-stop platform streamlines procurement, enabling you to focus on accelerating your assay development.