Anti-Snail Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - O95863

Core Product Specifications and Parameters

Biological Background: Snail Function and Localization

• Snail (SNAI1) is a zinc finger transcription factor essential for epithelial-mesenchymal transition (EMT), embryonic mesoderm formation, and cell migration. Related references: PMID:10655587, PMID:15647282

• Snail directly binds E-box sequences in the CDH1, CLDN7, and KRT8 promoters, and represses transcription by recruiting KDM1A histone demethylase, leading to decreased H3K4 methylation. Related references: PMID:20389281, PMID:20562920

• The N‑terminal SNAG domain of Snail acts as a histone H3 mimic, competing with histone H3 for binding to the KDM1A‑RCOR1 demethylase complex, thereby inhibiting demethylation at H3K4. Related references: PMID:20389281, PMID:21300290, PMID:23721412

• Snail participates in chromatin reorganization during EMT by recruiting LOXL2 to pericentromeric heterochromatin, where LOXL2 oxidizes histone H3 to repress transcription and release HP1α, facilitating a mesenchymal phenotype.

• Snail can associate with EGR1 and SP1 to mediate TPA‑induced upregulation of CDKN2B, and may independently activate the CDKN2B promoter. Related references: PMID:20121949

• Snail is predominantly localized in the nucleus but can also be found in the cytoplasm, consistent with its role as a transcription factor.

• Snail undergoes multiple post‑translational modifications, including phosphorylation, ADP‑ribosylation, glycosylation, and ubiquitination, which modulate its function and stability.

• Snail is widely expressed with highest levels in kidney, and is detected in both mesenchymal and epithelial cell lines.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the C‑terminal region (aa 200–267) of human Snail, which may avoid cross‑reactivity with other Snail family members.
• For Western blot, the predicted molecular weight is 29 kDa; consider using positive and negative controls such as Snail‑overexpressing versus knockdown cell lysates.
• IHC‑P and IF/ICC may require antigen retrieval and optimization of permeabilization conditions due to nuclear localization of the target.
• ELISA can be employed for quantitative detection, but cross‑reactivity with related proteins should be evaluated in the specific assay system.

CamelBio: Your One‑Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research institutions with a comprehensive portfolio of IVD raw materials, including validated antibodies, recombinant proteins, and ancillary reagents. For EMT research and cancer diagnostics, we offer optimized Snail antibodies and related products. Our sourcing expertise ensures reliable access to rare targets and bulk quantities, streamlining your development from concept to clinic.