Anti-SNAI1 (Snail) Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - O95863

Core Product Specifications and Parameters

Biological Background: Zinc finger protein SNAI1 (Snail) Function and Localization

• Zinc finger protein SNAI1 (Snail homolog 1) is a transcription factor involved in the induction of epithelial-mesenchymal transition (EMT), embryonic mesoderm formation and maintenance, growth arrest, survival, and cell migration.
  Related references: PMID:10655587, PMID:15647282, PMID:20389281
• Functions as transcriptional repressor by binding to E-boxes in the promoters of target genes (e.g., CDH1, CLDN7, KRT8) and recruiting histone demethylase KDM1A, leading to decreased H3K4 dimethylation.
  Related references: PMID:10655587, PMID:20389281, PMID:20562920
• The N-terminal SNAG domain competes with histone H3 for binding to the KDM1A/RCOR1 demethylase complex, inhibiting H3K4 demethylation in vitro.
  Related references: PMID:20389281, PMID:21300290, PMID:23721412
• During EMT, SNAI1 associates with LOXL2 to oxidize histone H3 at pericentromeric heterochromatin, facilitating chromatin reorganization and release of CBX5/HP1A, thereby promoting mesenchymal traits.
  Related references: PMID:16096638
• Can also activate transcription by cooperating with EGR1 and SP1 to up-regulate CDKN2B in response to TPA, and may independently stimulate the CDKN2B promoter.
  Related references: PMID:20121949
• Localizes to both the nucleus and cytoplasm.
• Widely expressed across tissues, with highest levels in kidney; present in mesenchymal and epithelial cell lines.
• Classified as a zinc-finger protein with key roles in development, DNA binding, and multiple post-translational modifications (ADP-ribosylation, phosphorylation, ubiquitination).

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 1-264 of human SNAI1, encompassing the full-length protein including the N-terminal SNAG domain and the zinc-finger regions, which may contribute to broad epitope recognition.
• For western blotting, a band around 29 kDa is expected; consider including nuclear and cytoplasmic fractionation controls given its dual localization.
• In IHC-P and IF/ICC experiments, primary localization is expected to be nuclear, but cytoplasmic staining may also be observed depending on cell state and EMT induction.
• Cross-reactivity with mouse and rat SNAI1 is indicated; validate specificity in your experimental system using appropriate controls.
• This polyclonal antibody may detect multiple isoforms or post-translationally modified forms; for critical applications, consider confirming results with orthogonal methods.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw-material partner, CamelBio provides diagnostic developers and research laboratories with seamless access to validated antibodies, bulk ancillary reagents, and customized sourcing solutions. This anti-SNAI1 polyclonal antibody supports EMT, cancer biology, and developmental studies, and can be integrated into larger assay development workflows. For projects requiring matched antibody pairs, rare-target reagents, or scalable supply, our supply network endeavors to simplify procurement from concept to clinic.