Anti-SMYD5 Rabbit Polyclonal Antibody for IF/ICC, ELISA - Q6GMV2

Core Product Specifications and Parameters

Biological Background: SMYD5 Function and Localization

• SMYD5 (SET and MYND domain-containing protein 5), also known as RRG1, RAI15, NN8-4AG, and ZMYND23, is a protein-lysine N-trimethyltransferase belonging to the SMYD family.
• It specifically trimethylates Lys-22 of the RPL40/eL40 ribosomal subunit, promoting translation elongation and protein synthesis. Related references: PMID:39048817 PMID:39103523
• SMYD5 may also function as a histone methyltransferase, catalyzing trimethylation of histone H3 at Lys-36 (H3K36me3) and histone H4 at Lys-20 (H4K20me3) in the context of histone octamers, though its activity on nucleosomes is uncertain in vivo. Related references: PMID:39048817 PMID:39103523
• In macrophages, SMYD5 associates with the NCoR corepressor complex to repress toll-like receptor 4 (TLR4)-target inflammatory genes, likely through deposition of H4K20me3 marks at gene promoters.
• It plays a critical role in embryonic stem (ES) cell self-renewal and differentiation by maintaining genome stability through heterochromatin formation and silencing of endogenous repetitive DNA elements via H4K20me3. Related references: PMID:28951459
• SMYD5 is also involved in the hypothermia response: mild hypothermia triggers its degradation, relieving H3K36me3-mediated repression at the SP1 neuroprotective gene promoter.
• Subcellular localization: Predominantly cytoplasmic.
• Contains a SET domain and a zinc-finger MYND domain; it binds zinc and S-adenosyl-L-methionine as a methyltransferase. Keywords include metal-binding, methyltransferase, and zinc-finger.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region between amino acids 300 and 400 of human SMYD5, which lies outside the annotated SET and MYND domains; consider validating reactivity against full-length protein or in systems expressing SMYD5.
• For immunofluorescence (IF/ICC), note that endogenous SMYD5 is reported to be cytoplasmic; ensure appropriate fixation and permeabilization protocols to detect native localization.
• ELISA can be used for antibody titering or to screen for SMYD5 in cell lysates; validate in the relevant sample system with appropriate controls.
• Cross-reactivity is reported for human only; if studying other species, verify sequence homology and perform experimental validation.

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