Anti-SMYD3 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9H7B4

Core Product Specifications and Parameters

Biological Background: SMYD3 Function and Localization

• SMYD3 is a histone-lysine N-methyltransferase that specifically methylates lysine 4 of histone H3, generating di- and trimethylation marks but not monomethylation. Related references: PMID:15235609, PMID:22419068
• It also catalyzes methylation of lysine 5 on histone H4. Related references: PMID:22419068
• Functions in transcriptional activation as part of an RNA polymerase complex and binds DNA containing the motifs 5'-CCCTCC-3' or 5'-GAGGGG-3'. Related references: PMID:15235609
• Localizes to both the cytoplasm and the nucleus.
• Expressed in skeletal muscle and testis, with overexpression frequently observed in colorectal and hepatocellular carcinomas.
• Contains a catalytic SET domain and a MYND-type zinc finger domain involved in protein-protein interactions.
• Subject to post-translational modifications including acetylation and phosphorylation; the gene undergoes alternative splicing.
• Classified under chromatin regulator and transferase keywords, highlighting its role in epigenetic control.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from amino acids 50–150 of human SMYD3 (NP_001161212.1); consider sequence alignment across species if cross-reactivity is critical.
• For Western blot, the predicted molecular weight is approximately 49 kDa; include appropriate molecular weight markers and use positive control lysates from tissues or cell lines known to express SMYD3 (e.g., skeletal muscle, testis, or certain cancer lines).
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), optimize fixation and permeabilization conditions, and validate staining with known positive and negative tissues/cells.
• As a polyclonal antibody, each new lot should be validated in your specific experimental system to ensure consistent performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with integrated access to IVD raw materials, supporting projects from concept to clinic. This SMYD3 polyclonal antibody complements our expanding portfolio for chromatin and cancer research applications. Beyond individual reagents, we offer validated antibody pairs, bulk ancillary reagents, and custom sourcing for rare-target raw materials—backed by comprehensive technical and consulting services to streamline your diagnostic development.