Anti-SMYD2 Polyclonal Antibody for WB/IF/ELISA - Q9NRG4

Core Product Specifications and Parameters

Biological Background: SMYD2 Function and Localization

• SMYD2 is an N-lysine methyltransferase that methylates both histones and non-histone proteins, including p53/TP53 and RB1.
• It specifically trimethylates histone H3 at lysine 4 (H3K4me3), a modification associated with active transcription.
• Methyltransferase activity on p53/TP53 is higher than on histones; SMYD2 monomethylates p53 at Lys-370, reducing its DNA-binding and transcriptional activity.
• SMYD2 also monomethylates RB1 at Lys-860, potentially regulating tumor suppressor functions.
• The methyltransferase activity of SMYD2 requires interaction with HSP90alpha.
• SMYD2 contains a SET domain and a MYND-type zinc finger, classifying it as a member of the SMYD family of methyltransferases.
• The protein localizes to both the cytoplasm (cytosol) and the nucleus.
• SMYD2 is associated with chromatin regulation, transcription regulation, and metal/zinc binding based on UniProt keywords.

Experimental Guidance and Technical Tips

• The immunogen encompasses the N-terminal region (aa 1-270) of human SMYD2, which may allow recognition of multiple isoforms or splice variants.
• For Western blot (WB), consider validating the antibody using human and mouse cell lysates, and expect a band around 50 kDa; optimize protein loading and detection conditions.
• In immunofluorescence (IF/ICC), test appropriate fixation and permeabilization protocols to visualize SMYD2 subcellular localization (cytoplasmic and/or nuclear).
• For ELISA, consider using purified recombinant SMYD2 or cell extracts as coating antigen, and perform a titration to determine optimal antibody concentration.
• As a polyclonal antibody, it may exhibit batch-to-batch variability; include positive and negative controls in each experiment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to a comprehensive portfolio of IVD raw materials, technical services, and consulting. This anti-SMYD2 polyclonal antibody supports epigenetic and cancer biology research, and CamelBio can assist with sourcing validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and even rare target raw materials to accelerate your assay development from concept to clinic.